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PMID: 372560 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Involvement of DNA gyrase in replication and transcription of bacteriophage T7 DNA.

Journal of virology ·Vol. 29 ·No. 2 ·1979-02-00 ·Pages 529-35

De Wyngaert M, Hinkle DC

Abstract

Growth of bacteriophage T7 is inhibited by the antibiotic coumermycin A1, an inhibitor of the Escherichia coli DNA gyrase. Since growth of the phage is insensitive to the antibiotic in strains containing a coumermycin-resistant DNA gyrase, this enzyme appears to be required for phage growth. We have investigated the effect of coumermycin on the kinetics of DNA, RNA, and protein synthesis during T7 infection. DNA synthesis is completely inhibited by the antibiotic. In addition, coumermycin significantly inhibits transcription of late but not early genes. Thus, E. coli DNA gyrase may play an important role in transcription as well as in replication of T7 DNA.

MeSH Terms
Anti-Bacterial Agents/pharmacology Coliphages/metabolism Coumarins/pharmacology DNA Replication DNA, Bacterial/metabolism DNA, Superhelical/metabolism DNA, Viral/biosynthesis Drug Resistance, Microbial Escherichia coli/enzymology,genetics Mutation Pyrroles/pharmacology RNA, Viral/biosynthesis Transcription, Genetic/drug effects Viral Proteins/biosynthesis
Chemicals
Anti-Bacterial Agents Coumarins DNA, Bacterial DNA, Superhelical DNA, Viral Pyrroles RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
De Wyngaert M
Hinkle D C
References (26)
26 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.29.2.529-535.1979
Published
1979-02-00
Pages
529-35
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353186
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.29.2.529-535.1979 PMC Full Text View journal details
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