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PMID: 373461 Published · ppublish English Journal Article

Kinetics of prostaglandin production in various inflammatory lesions, measured in draining lymph.

The American journal of pathology ·Vol. 95 ·No. 1 ·1979-04-00 ·Pages 225-38

Johnston MG, Hay JB, Movat HZ

Abstract

Efferent lymph was collected over long periods via catheters surgically placed in popliteal and prefemoral lymph nodes of sheep. Prostaglandin (PG) E and F equivalents were measured with a radioimmunoassay. After stimulation with heat-killed Escherichia coli, PG levels rose dramatically in the efferent lymph but were undetectable in the contralateral control lymphatics or in the systemic circulation. When E coli were infused directly into a lymph node, the PG levels in the effluent lymph were inhibited with indomethacin. Carrageenan, delayed hypersensitivity, and lymphocyte transfer reactions were also studied. In the classic acute inflammations (caused by E coli and carrageenan) the PG levels rose early in the response (first 4 to 6 hours) compared with delayed production in the immune reactions. With PPD, PG levels peaked between 10 and 20 hours after injections, while PG rose 127 hours after allogeneic lymphocytes were injected. These results are discussed in relation to the role of PG in inflammation, and the use of the sheep lymphatic model in PG research is emphasized.

MeSH Terms
Animals Capillary Permeability Carrageenan/pharmacology Escherichia coli/immunology Female Hypersensitivity/immunology Immunization, Passive Indomethacin/pharmacology Inflammation/immunology Kinetics Lymph/immunology Male Prostaglandins/biosynthesis Prostaglandins E/biosynthesis Prostaglandins F/biosynthesis Sheep
Chemicals
Prostaglandins Prostaglandins E Prostaglandins F Carrageenan Indomethacin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Johnston M G
Hay J B
Movat H Z
References (38)
38 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1979-04-00
Pages
225-38
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC2042292
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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