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PMID: 3748821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A left-handed (Z) conformation of poly(dA-dC).poly(dG-dT) induced by polyamines.

Nucleic acids research ·Vol. 14 ·No. 16 ·1986-08-26 ·Pages 6721-33

Thomas TJ, Messner RP

Abstract

Blocks of potential Z-DNA forming alternating purine-pyrimidine (APP) sequences are widely dispersed in native DNAs. We have studied the effects of naturally occurring polyamines on the conformation of a synthetic APP sequence, poly(dA-dC).poly(dG-dT) by circular dichroism spectroscopy. In the presence of micromolar concentrations of spermidine (125 microM) and spermine (16 microM), this polymer undergoes B to Z transition in low ionic strength (2 mM Na+) buffers. The concentration of polyamines required for B to Z transition increases with Na+ in the buffer and a straight line is obtained on plotting ln[Na+] vs. ln [spermidine 3+]. However, at concentrations of polyamines higher than those necessary to induce B to Z transition, Z-DNA converts to psi-DNA, an ordered, twisted, tight packing arrangement of the double helix. These results suggest a pathway for the transient formation of Z-DNA segments in vivo by interaction of the ubiquitous polyamines with naturally occurring blocks of APP sequences.

MeSH Terms
Base Sequence Circular Dichroism Nucleic Acid Conformation Osmolar Concentration Polyamines Polydeoxyribonucleotides Putrescine Spermidine Spermine
Chemicals
Polyamines Polydeoxyribonucleotides Spermine poly(dA-dC).poly(dG-dT) Spermidine Putrescine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thomas T J
Messner R P
References (44)
44 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.16.6721
Published
1986-08-26
Pages
6721-33
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311676
Subset
IM
Grants
NIADDK NIH HHS · AM 07437-03 · United States
External Links
PubMed source DOI: 10.1093/nar/14.16.6721 PMC Full Text View journal details
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