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PMID: 375225 Published · ppublish English Journal Article

Enzymatic insertion of purine bases into depurinated DNA in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 3 ·1979-03-00 ·Pages 1089-93

Livneh Z, Elad D, Sperling J

Abstract

An enzymatic activity that inserts purines into depurinated DNA was found in a soluble enzyme extract of Escherichia coli. This activity brings about the insertion of adenine and guanine into the appropriate apurinic sites in double-stranded DNA by using the corresponding deoxyribonucleoside triphosphates as the purine donors. Magnesium ions are required for this activity, it is inhibited by caffeine, and it does not act on depurinated single-stranded DNA. The insertion activity described here may represent a step in a repair mechanism, "base-insertion repair," whereby apurinic sites (which may occur in double-stranded DNA either due to the removal of damaged purines with specific glycosylases or by spontaneous depurination) are directly filled with the correct missing purine base.

MeSH Terms
Caffeine/pharmacology Coliphages DNA Repair DNA, Viral/metabolism Deoxyribonucleotides/metabolism Escherichia coli/metabolism Kinetics Magnesium/pharmacology Purines/metabolism
Chemicals
DNA, Viral Deoxyribonucleotides Purines Caffeine Magnesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Livneh Z
Elad D
Sperling J
References (21)
21 references, click to expand
  1. FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES.
    Biochem J. 1963 Oct;89:127-38 PMID: 14097355
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Repair of depurinated DNA in vitro by enzymes purified from human lymphoblasts.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):794-8 PMID: 273243
  4. A new endonuclease from Escherichia coli acting at apurinic sites in DNA.
    J Biol Chem. 1977 May 10;252(9):2808-14 PMID: 16002
  5. Human endonuclease specific for apurinic/apyrimidinic sites in DNA. Partial purification and characterization of multiple forms from placenta.
    J Biol Chem. 1977 Feb 25;252(4):1235-42 PMID: 14146
  6. Properties of 3-methyladenine-DNA glycosylase from Escherichia coli.
    Biochemistry. 1978 May 30;17(11):2110-8 PMID: 352392
  7. Caffeine.
    Mutat Res. 1977;47(1):1-52 PMID: 342928
  8. Two enzymes are required from strand incision in repair of alkylated DNA.
    Nature. 1977 Oct 27;269(5631):829-32 PMID: 927511
  9. An endonuclease from calf liver specific for apurinic sites in DNA.
    Biochemistry. 1977 Apr 5;16(7):1370-7 PMID: 849421
  10. In vitro repair of apurinic sites in DNA.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2273-5 PMID: 4601584
  11. Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases.
    J Biol Chem. 1972 Jan 10;247(1):241-8 PMID: 4336040
  12. A mammalian endonuclease specific for apurinic sites in double-stranded deoxyribonucleic acid. I. Purification and general properties.
    J Biol Chem. 1974 Mar 10;249(5):1530-5 PMID: 4206354
  13. Rate of depurination of native deoxyribonucleic acid.
    Biochemistry. 1972 Sep 12;11(19):3610-8 PMID: 4626532
  14. The enzymatic release of O6-methylguanine and 3-methyladenine from DNA reacted with the carcinogen N-methyl-N-nitrosourea.
    Proc Natl Acad Sci U S A. 1974 May;71(5):2022-6 PMID: 4600266
  15. Structure and synthesis of a lipid-containing bacteriophage. Preparation of virus and localization of the structural proteins.
    Eur J Biochem. 1974 Dec 16;50(1):1-14 PMID: 4452350
  16. The intermediate in the degradation of DNA alkylated with a monofunctional alkylating agent.
    Biochim Biophys Acta. 1970 Jul 16;213(1):14-25 PMID: 4992323
  17. Purification of Escherichia coli endonuclease specific for apurinic sites in DNA.
    J Biol Chem. 1975 Oct 25;250(20):8214-9 PMID: 1100631
  18. Human endonuclease activity for DNA apurinic sites.
    Nature. 1975 Dec 11;258(5535):544-7 PMID: 1196390
  19. DNA of bacteriophage PM2: a closed circular double-stranded molecule.
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164-8 PMID: 5260915
  20. Structure of the DNA of bacteriophage phiX174. VII. Methylation.
    J Mol Biol. 1970 Oct 28;53(2):251-9 PMID: 5493279
  21. The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.
    J Mol Biol. 1969 Oct 28;45(2):237-63 PMID: 5367027
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.3.1089
Published
1979-03-00
Pages
1089-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383194
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.3.1089 PMC Full Text View journal details
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