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PMID: 375237 Published · ppublish English Comparative Study Journal Article

Strand exchange in site-specific recombination.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 3 ·1979-03-00 ·Pages 1363-7

Enquist LW, Nash H, Weisberg RA

Abstract

The site-specific recombination system of phage lambda promotes crossovers at its attachment site (att). In this report we show that when phage are crossed in conditions where only the site-specific recombination system is active, a low frequency of crossovers can also be detected in a region that is close to but does not contain att. These crossovers require the phage int gene, the host hip gene, and the integrity of att. They are not detected if one of the parents carries a substitution of a heterologous attachment site (attB instead of attP). To explain these findings we suggest that site-specific recombination can proceed by exchange of single strands between the participating chromosomes at att and migration of the resulting junction outside of att.

MeSH Terms
Alleles Coliphages/metabolism Crossing Over, Genetic Escherichia coli/metabolism Genotype Recombination, Genetic Species Specificity
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Enquist L W
Nash H
Weisberg R A
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.3.1363
Published
1979-03-00
Pages
1363-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383251
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.3.1363 PMC Full Text View journal details
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