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PMID: 3753440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification of cathepsin B by a new form of affinity chromatography.

The Biochemical journal ·Vol. 235 ·No. 3 ·1986-05-01 ·Pages 731-4

Rich DH, Brown MA, Barrett AJ

Abstract

Human cathepsin B was purified by affinity chromatography on the semicarbazone of Gly-Phe-glycinal linked to Sepharose 4B, with elution by 2,2'-dipyridyl disulphide at pH 4.0. The product obtained in high yield by the single step from crude starting material was 80-100% active cathepsin B. The possibility that this new form of affinity chromatography may be of general usefulness in the purification of cysteine proteinases is discussed.

MeSH Terms

2,2'-Dipyridyl/analogs & derivatives Cathepsin B Cathepsins/isolation & purification Chromatography, Affinity/methods Disulfides Electrophoresis, Polyacrylamide Gel Humans Oligopeptides Semicarbazones Sepharose

Chemicals

Disulfides Oligopeptides Semicarbazones glycyl-phenylalanyl-glycine-semicarbazone 2,2'-dipyridyl disulfide 2,2'-Dipyridyl Sepharose Cathepsins Cathepsin B

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rich D H

Brown M A

Barrett A J

References (15)

15 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2350731

Published

1986-05-01

Pages

731-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1146748

Subset

External Links

PubMed source DOI: 10.1042/bj2350731 PMC Full Text View journal details

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Purification of cathepsin B by a new form of affinity chromatography.

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