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PMID: 3754960 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A cDNA clone of the hnRNP C proteins and its homology with the single-stranded DNA binding protein UP2.

Nucleic acids research ·Vol. 14 ·No. 10 ·1986-05-27 ·Pages 4077-94

Lahiri DK, Thomas JO

Abstract

A cDNA clone which expresses a protein that cross-reacts immunologically with the human C1 and C2 hnRNP core proteins has been isolated. The clone was selected by a sensitive immunochemical assay employing an avidin-biotin complex for detection, and identified as a clone for the hnRNP C proteins by a highly sensitive antibody select assay that is described here. The clone contains 677 nucleotides, and, as shown by northern blotting, is derived from a 1.5 Kb poly(A)+ mRNA. There are regions of strong homology between the human and mouse genes, weak homology is seen with chicken DNA, and very little, if any, homology can be detected with Drosophila, Artemia, sea urchin, or yeast DNAs. Two peptides (a total of 24 amino acids) of the calf thymus single-stranded DNA binding protein UP2 show perfect homology with the deduced amino acid sequence of the clone, suggesting that UP2 is related to the hnRNP C proteins. There is also a region that has a sequence very similar to two regions of the single-stranded DNA binding protein UP1 that contain proposed DNA binding sites.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cattle Chickens Cloning, Molecular Cross Reactions DNA/analysis DNA-Binding Proteins/analysis,genetics Electrophoresis, Polyacrylamide Gel Heterogeneous-Nuclear Ribonucleoprotein Group C Heterogeneous-Nuclear Ribonucleoproteins Humans Molecular Weight Ribonucleoproteins/genetics
Chemicals
C1 HNRNP DNA-Binding Proteins HNRNPC protein, human Heterogeneous-Nuclear Ribonucleoprotein Group C Heterogeneous-Nuclear Ribonucleoproteins Hnrnpc protein, mouse Ribonucleoproteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lahiri D K
Thomas J O
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.10.4077
Published
1986-05-27
Pages
4077-94
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339847
Subset
IM
Grants
NIGMS NIH HHS · GM28747 · United States
Databases
GENBANK
X03910
External Links
PubMed source DOI: 10.1093/nar/14.10.4077 PMC Full Text View journal details
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