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PMID: 3755819 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural and functional analysis of an alternatively spliced chicken TK messenger RNA.

Nucleic acids research ·Vol. 14 ·No. 15 ·1986-08-11 ·Pages 6281-97

Merrill GF, Tufaro FD

Abstract

The nucleotide sequence of independent cDNA clones revealed that TK transcripts can undergo alternative splicing within the 3' nontranslated portion of the message. The most abundant mRNA is 2.1 kb in length and is colinear with the underlying genomic sequence for at least the first 1052 bases of its 3' nontranslated region. A less abundant mRNA, 1.3 kb in length, differs from the larger mRNA in that an 863 base intron is spliced from the 3' nontranslated region. The possibility that removal of the alternatively spliced intron allows the small mRNA to persist in postreplicative cells was investigated in two ways. First, the levels of large and small TK mRNA in tissues expressing differential growth rates was determined. Second, the pattern of TK enzyme expression during differentiation was analyzed in muscle cells transformed with recombinant TK genes lacking the 3' nontranslated intron. Both lines of experimentation indicated that the small TK mRNA was as dependent as the large TK mRNA on the replicative state of the cell.

MeSH Terms
Animals Base Sequence Cell Cycle Cell Differentiation Chickens DNA/genetics L Cells/metabolism Mice Muscles/metabolism RNA Splicing RNA, Messenger/genetics Recombinant Proteins/biosynthesis Thymidine Kinase/biosynthesis,genetics Transformation, Genetic
Chemicals
RNA, Messenger Recombinant Proteins DNA Thymidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Merrill G F
Tufaro F D
References (29)
29 references, click to expand
  1. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  2. DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.
    Exp Cell Res. 1963 Aug;31:297-312 PMID: 14056989
  3. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  4. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  5. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  8. Isolation of the chicken thymidine kinase gene by plasmid rescue.
    Nature. 1980 May 22;285(5762):207-10 PMID: 6246445
  9. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland.
    Cell. 1980 Aug;21(1):179-87 PMID: 6157477
  10. Differentiation of thymidine kinase deficient mouse myoblasts in the presence of 5'-bromodeoxyuridine.
    Exp Cell Res. 1980 Sep;129(1):191-9 PMID: 7428811
  11. Control of mouse myoblast commitment to terminal differentiation by mitogens.
    J Supramol Struct. 1980;14(4):483-98 PMID: 6454029
  12. Enhancing the efficiency of DNA-mediated gene transfer in mammalian cells.
    Somatic Cell Genet. 1981 Sep;7(5):603-16 PMID: 7292260
  13. Control of dihydrofolate reductase messenger ribonucleic acid production.
    Mol Cell Biol. 1981 Nov;1(11):961-71 PMID: 6180296
  14. Mutational analysis of the cloned chicken thymidine kinase gene.
    J Mol Appl Genet. 1983;2(2):191-200 PMID: 6875426
  15. Isolation and preliminary characterization of the Chinese hamster thymidine kinase gene.
    Mol Cell Biol. 1983 Oct;3(10):1815-23 PMID: 6227808
  16. In vitro and in vivo analysis of the control of dihydrofolate reductase gene transcription in serum-stimulated mouse fibroblasts.
    J Cell Physiol. 1984 Jan;118(1):79-86 PMID: 6690454
  17. Post-transcriptional regulation of the chicken thymidine kinase gene.
    Nucleic Acids Res. 1984 Feb 10;12(3):1427-46 PMID: 6199739
  18. Expression of complete chicken thymidine kinase gene inserted in a retrovirus vector.
    Mol Cell Biol. 1984 Apr;4(4):749-54 PMID: 6325895
  19. Synthesis of two mRNAs by utilization of alternate polyadenylation sites: expression of SV40-mouse immunoglobulin mu chain gene recombinants in Cos monkey cells.
    EMBO J. 1984 Apr;3(4):689-99 PMID: 6327285
  20. Dihydrofolate reductase gene expression in cultured mouse cells is regulated by transcript stabilization in the nucleus.
    J Cell Biol. 1984 Jul;99(1 Pt 1):180-7 PMID: 6736126
  21. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  22. The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5' end in different developmental stages.
    Cell. 1983 May;33(1):125-33 PMID: 6432335
  23. Genetic and physical analysis of the chicken tk gene.
    Mol Cell Biol. 1984 Sep;4(9):1769-76 PMID: 6092937
  24. tk Enzyme expression in differentiating muscle cells is regulated through an internal segment of the cellular tk gene.
    Mol Cell Biol. 1984 Sep;4(9):1777-84 PMID: 6493233
  25. Levels of c-myc oncogene mRNA are invariant throughout the cell cycle.
    Nature. 1985 Mar 28-Apr 3;314(6009):363-6 PMID: 3982504
  26. Transcriptional regulation of mouse dihydrofolate reductase in the cell cycle.
    J Biol Chem. 1985 Jun 25;260(12):7675-80 PMID: 2987264
  27. Faithful cell-cycle regulation of a recombinant mouse histone H4 gene is controlled by sequences in the 3'-terminal part of the gene.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4389-93 PMID: 3925455
  28. Involvement of the 5'-leader sequence in coupling the stability of a human H3 histone mRNA with DNA replication.
    Proc Natl Acad Sci U S A. 1986 Feb;83(4):981-5 PMID: 3081892
  29. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.15.6281
Published
1986-08-11
Pages
6281-97
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311636
Subset
IM
Grants
NIA NIH HHS · AG05388 · United States
NIGMS NIH HHS · GM34432 · United States
Databases
GENBANK
X04352, X04353
External Links
PubMed source DOI: 10.1093/nar/14.15.6281 PMC Full Text View journal details
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