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PMID: 3758028 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11.

The EMBO journal ·Vol. 5 ·No. 8 ·1986-08-00 ·Pages 1831-7

Mason IJ, Murphy D, Münke M, Francke U, Elliott RW, Hogan BL

Abstract

SPARC is a Mr 43,000 secreted, acidic, cysteine-rich glycoprotein homologous to 43K bovine endothelial 'culture shock' protein. We show here that it is encoded by a single gene localized to the central region of mouse chromosome 11. During development SPARC mRNA is expressed at higher levels in all the extra-embryonic tissues than in the fetus. Highest levels are found in the parietal endoderm, while visceral endoderm has approximately 6-fold less. This differential expression is also seen in F9 teratocarcinoma cells treated with retinoic acid under conditions in which they give rise to either parietal or visceral endoderm. The 20-fold increase seen during differentiation into parietal endoderm is due, at least in part, to an increase in gene transcription. We also report SPARC expression in a variety of adult tissues and cultured cells, and present evidence that a decrease accompanies the transformation of fibroblast cell lines.

MeSH Terms
Animals Cell Differentiation Cell Line Chromosome Mapping Cricetinae Embryo, Mammalian Female Fetus Genes Glycoproteins/genetics Hybrid Cells/cytology Mice Mice, Inbred C3H Mice, Inbred CBA Nucleic Acid Hybridization Osteonectin Pregnancy RNA, Messenger/genetics Teratoma/pathology Transcription, Genetic
Chemicals
Glycoproteins Osteonectin RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mason I J
Murphy D
Münke M
Francke U
Elliott R W
Hogan B L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-08-00
Pages
1831-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167048
Subset
IM
Grants
NIGMS NIH HHS · GM26105 · United States
NIGMS NIH HHS · GM28464 · United States
External Links
PubMed source PMC Full Text View journal details
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