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PMID: 3766942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid preparation of bacteriophage DNA for sequence analysis in sets of 96 clones, using filtration.

Analytical biochemistry ·Vol. 156 ·No. 2 ·1986-08-01 ·Pages 406-12

Eperon IC

Abstract

A method is described for the preparation of single-stranded DNA from clones in bacteriophage M13 vectors. This procedure allows multiples of 96 clones to be processed at once, utilizing filtration to remove host cells and simplifying the treatment of bacteriophage pellets. The DNA produced can be used for sequencing of mutagenesis.

MeSH Terms
Bacteriophages/analysis Base Sequence DNA, Viral/analysis,isolation & purification Electrophoresis, Polyacrylamide Gel Filtration Mutation
Chemicals
DNA, Viral
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Eperon I C
Article Info
Journal
Analytical biochemistry
Abbr.
Anal Biochem
ISSN
0003-2697
DOI
10.1016/0003-2697(86)90273-3
Published
1986-08-01
Pages
406-12
Language
English
Region
United States
NLM ID
0370535
Subset
IM
External Links
PubMed source DOI: 10.1016/0003-2697(86)90273-3 View journal details
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