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PMID: 3767361 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Evaluation of methods for enumeration of Vibrio parahaemolyticus from seafood.

Applied and environmental microbiology ·Vol. 52 ·No. 3 ·1986-09-00 ·Pages 583-5

Karunasagar I, Venugopal MN, Karunasagar I, Segar K

Abstract

The efficiency of several enrichment broths in recovering Vibrio parahaemolyticus inoculated into fish homogenates was studied. Recovery by the most probable number technique was very low in all the broths, while direct plating on thiosulfate citrate bile salt sucrose agar yielded better recovery. A decrease in the enrichment time to 8 from 18 h did not improve recovery. At concentrations exceeding 2.5 micrograms/ml, polymyxin was inhibitory to V. parahaemolyticus.

MeSH Terms
Animals Culture Media Decapoda/microbiology Fishes/microbiology Food Microbiology Vibrio parahaemolyticus/growth & development,isolation & purification
Chemicals
Culture Media
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Karunasagar I
Venugopal M N
Karunasagar I
Segar K
References (5)
5 references, click to expand
  1. A survey on the occurrence of Vibrio parahaemolyticus on fish and shellfish, marketed in the Netherlands.
    J Hyg (Lond). 1970 Jun;68(2):189-96 PMID: 5270202
  2. Ecology of Vibrio parahaemolyticus in Chesapeake Bay.
    J Bacteriol. 1973 Jan;113(1):24-32 PMID: 4567138
  3. An evaluation of the efficiency of enrichment media in the isolation process for Vibrio parahaemolyticus.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Apr;256(4):456-65 PMID: 6377759
  4. Optimal enrichment time for isolation of Vibrio parahaemolyticus from seafood.
    Appl Environ Microbiol. 1983 Nov;46(5):1234-5 PMID: 6651300
  5. Recovery of chill-stressed Vibrio parahaemolyticus from oysters with enrichment broths supplemented with magnesium and iron salts.
    Appl Environ Microbiol. 1980 Jan;39(1):179-85 PMID: 7356314
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.52.3.583-585.1986
Published
1986-09-00
Pages
583-5
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC203578
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.52.3.583-585.1986 PMC Full Text View journal details
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