Further studies on the mode of action of the heme-controlled translational inhibitor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 4 ·1979-04-00 ·Pages 1741-5

de Haro C, Ochoa S

Abstract

We have isolated [de Haro, C. & Ochoa, S. (1978) Proc. Natl. Acad. Sci. USA 75, 2713-2716] a protein factor (eIF-2 stimulating protein, ESP) that is essential for formation of ternary and 40S initiation complexes by the eukaryotic polypeptide chain initiation factor 2 (eIF-2) at the low concentrations of eIF-2 present in reticulocyte lysates. The fact that stimulation of complex formation by ESP is virtually abolished when the small (38,000 daltons) subunit of eIF-2 is phosphorylated by ATP in the presence of eIF-2 kinase (heme-controlled inhibitor, HCI) is consistent with the notion that HCI inhibits translation in lysates by blocking the interaction of eIF-2 with ESP. Our present work, with highly purified eIF-2 and ESP, has additionally established that, unlike phosphorylation of the small subunit, phosphorylation of the middle (52,000 daltons) subunit of eIF-2, which does not lead to translational inhibition in lysates, does not affect eIF-2-ESP interaction. This provides further support for our model of translational inhibition by HCI.

MeSH Terms

Animals Bacterial Proteins/isolation & purification,physiology Escherichia coli/metabolism Heme/physiology Kinetics Molecular Weight Peptide Chain Initiation, Translational Peptide Initiation Factors/isolation & purification,metabolism Protein Biosynthesis RNA, Transfer/metabolism Rabbits Reticulocytes/metabolism Ribosomes/metabolism

Chemicals

Bacterial Proteins Peptide Initiation Factors Heme RNA, Transfer

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

de Haro C

Ochoa S

References (21)

21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.4.1741

Published

1979-04-00

Pages

1741-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC383466

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.4.1741 PMC Full Text View journal details

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Further studies on the mode of action of the heme-controlled translational inhibitor.

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