Home LiteratureArticle Details
PMID: 3774537 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A subpopulation of spinach chloroplast tRNA genes does not require upstream promoter elements for transcription.

Nucleic acids research ·Vol. 14 ·No. 19 ·1986-10-10 ·Pages 7541-56

Gruissem W, Elsner-Menzel C, Latshaw S, Narita JO, Schaffer MA, Zurawski G

Abstract

We have identified a class of spinach plastid tRNA genes which do not require 5' upstream promoter elements for their expression in a chloroplast transcription system. The 5' DNA sequences flanking the trnR1 and trnS1 coding regions have little or no homology to previously characterized chloroplast promoter sequences. The deletion of the 5' DNA sequences from these genes to positions close to the start of the coding regions has little effect on their transcription in vitro. In addition, a synthetic DNA fragment homologous to the 5' region of trnS1 does not support the transcription of the promoter (-) trnM2 mutant 51 in a promoter/trnM2-51 fusion assay. In a dicistronic construct the wild type trnS1 gene does not support transcription of trnS1 transcription occurs immediately following the 3' end of the coding region. Both trnS1 and trnR1 compete with trnM2 for the same chloroplast RNA polymerase and/or common transcription factors.

MeSH Terms
Base Sequence Chloroplasts/metabolism Chromosome Deletion DNA/analysis Mutation Promoter Regions, Genetic RNA, Transfer/genetics Transcription, Genetic
Chemicals
DNA RNA, Transfer
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gruissem W
Elsner-Menzel C
Latshaw S
Narita J O
Schaffer M A
Zurawski G
References (15)
15 references, click to expand
  1. Identification and mutational analysis of the promoter for a spinach chloroplast transfer RNA gene.
    EMBO J. 1985 Jul;4(7):1637-44 PMID: 2992936
  2. In vitro synthesis and processing of a maize chloroplast transcript encoded by the ribulose 1,5-bisphosphate carboxylase large subunit gene.
    Mol Cell Biol. 1985 Oct;5(10):2733-45 PMID: 2874479
  3. Chloroplast promoters from higher plants.
    Nucleic Acids Res. 1985 Nov 11;13(21):7543-9 PMID: 4069994
  4. Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of photosystem II and for tRNASer (UGA).
    Nucleic Acids Res. 1984 Dec 11;12(23):8819-34 PMID: 6096808
  5. Biosynthesis of chloroplast transfer RNA in a spinach chloroplast transcription system.
    Cell. 1983 Dec;35(3 Pt 2):815-28 PMID: 6652686
  6. An in vitro system for accurate transcription initiation of chloroplast protein genes.
    Nucleic Acids Res. 1985 Feb 25;13(4):1283-302 PMID: 2860636
  7. Internal control regions for transcription of eukaryotic tRNA genes.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6657-61 PMID: 6947245
  8. Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
    Nature. 1981 Dec 17;294(5842):626-31 PMID: 7312050
  9. Evidence for two RNA polymerase activities in Euglena gracilis chloroplasts.
    J Biol Chem. 1984 Dec 10;259(23):14880-7 PMID: 6094579
  10. A sixth subunit of ATP synthase, an F(0) component, is encoded in the pea chloroplast genome.
    EMBO J. 1986 Feb;5(2):217-22 PMID: 16453667
  11. Structure and cotranscription of tobacco chloroplast genes for tRNAGlu(UUC), tRNATyr(GUA) and tRNAAsp(GUC).
    Nucleic Acids Res. 1985 Feb 25;13(4):1045-56 PMID: 4000934
  12. Transcription of E. coli and Euglena chloroplast tRNA gene clusters and processing of polycistronic transcripts in a HeLa cell-free system.
    Cell. 1982 Aug;30(1):81-92 PMID: 6181897
  13. Selective in vitro transcription of chloroplast genes.
    J Cell Biochem. 1983;22(1):31-46 PMID: 6421827
  14. Analysis of promoter regions for the spinach chloroplast rbcL, atpB and psbA genes.
    EMBO J. 1985 Dec 16;4(13A):3375-83 PMID: 2868888
  15. DNA sequence requirements for the accurate transcription of a protein-coding plastid gene in a plastid in vitro system from mustard (Sinapis alba L.).
    EMBO J. 1984 Aug;3(8):1697-704 PMID: 16453540
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.19.7541
Published
1986-10-10
Pages
7541-56
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311779
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.19.7541 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2