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PMID: 3782463 Published · ppublish English Comparative Study Journal Article

Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile.

Journal of clinical microbiology ·Vol. 24 ·No. 6 ·1986-12-00 ·Pages 991-4

Delmée M, Laroche Y, Avesani V, Cornelis G

Abstract

A typing scheme for Clostridium difficile based on slide agglutination with rabbit antisera was previously described. It allows the differentiation of 10 serogroups designated A, B, C, D, F, G, H, I, K, and X. We studied the correlation between serogrouping and polyacrylamide gel electrophoresis (PAGE) of whole-cell proteins. A total of 202 isolates from different sources were analyzed by PAGE after ultrasonic disintegration of cells from an 18-h liquid culture and treatment with sodium dodecyl sulfate and 2-mercaptoethanol. A total of 21 different patterns were observed. The reference strains from the 10 serogroups showed different profiles. For each serogroup except A, the patterns obtained with the clinical isolates were identical to the patterns obtained with the reference strains. For the 48 strains belonging to serogroup A, 12 different profiles were observed. Five of these involved strains isolated from patients with antibiotic-associated diarrhea. Typing by sodium dodecyl sulfate-PAGE thus correlates with serogrouping. In addition, it allows discrimination within the heterogeneous serogroup A.

MeSH Terms
Agglutination Tests Bacterial Proteins/analysis Clostridium/analysis,classification Diarrhea/microbiology Electrophoresis, Polyacrylamide Gel Humans Serotyping
Chemicals
Bacterial Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Delmée M
Laroche Y
Avesani V
Cornelis G
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22 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.24.6.991-994.1986
Published
1986-12-00
Pages
991-4
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC269085
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.24.6.991-994.1986 PMC Full Text View journal details
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