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PMID: 378937 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Penicillin selection of Escherichia coli deoxyribonucleic acid repair mutants.

Journal of bacteriology ·Vol. 138 ·No. 3 ·1979-06-00 ·Pages 779-82

Sancar A, Rupert CS

Abstract

A rapid method has been developed for isolation of ultraviolet-sensitive mutants of Escherichia coli, by inducing delay in the growth and/or division of repair-deficienct cells with low fluences of far-ultraviolet radiation, and killing with penicillin the repair-proficient cells, which continue to grow and divide. With this technique, we have achieved about a 3,000-fold enrichment for photoreactivation less (phr) cells and have isolated and characterized three phr mutants.

MeSH Terms
DNA Repair DNA, Bacterial/genetics,radiation effects Escherichia coli/drug effects,genetics,radiation effects Light Mutation Penicillins/pharmacology Ultraviolet Rays
Chemicals
DNA, Bacterial Penicillins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sancar A
Rupert C S
References (14)
14 references, click to expand
  1. CORRELATION BETWEEN GENETIC TRANSFORMABILITY AND NON-PHOTOREACTIVABILITY IN BACILLUS SUBTILIS.
    J Bacteriol. 1964 Jun;87:1295-303 PMID: 14188705
  2. Mutants of phage T4 with increased sensitivity to ultraviolet.
    Virology. 1963 Jan;19:66-71 PMID: 13952844
  3. A method for selecting radiation-sensitive mutants of Escherichia coli.
    Genetics. 1962 Sep;47:1219-24 PMID: 13955138
  4. A small and inexpensive ultraviolet dose-rate meter useful in biological experiements.
    Radiat Res. 1961 Apr;14:394-403 PMID: 13789181
  5. Studies in the physiology and biochemistry of penicillin-induced spheroplasts of Escherichia coli.
    J Bacteriol. 1958 Dec;76(6):612-7 PMID: 13610799
  6. Mechanism of action of penicillin.
    J Bacteriol. 1957 Jan;73(1):144 PMID: 13405877
  7. Growth, respiration, and nucleic acid synthesis in ultraviolet-irradiated and in photoreactivated Escherichia coli.
    J Bacteriol. 1953 Mar;65(3):252-62 PMID: 13034732
  8. Effects of ultraviolet radiation on respiration and growth in radiation-resistant and radiation-sensitive strains of Escherichia coli B.
    J Bacteriol. 1969 Sep;99(3):815-23 PMID: 4905539
  9. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  10. Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli.
    J Mol Biol. 1971 Oct 14;61(1):25-44 PMID: 4947693
  11. Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control.
    J Bacteriol. 1970 Oct;104(1):34-44 PMID: 4919751
  12. Autolytic enzymes and cell division of Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):419-29 PMID: 4896021
  13. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA.
    Genetics. 1966 Jun;53(6):1119-36 PMID: 5335128
  14. Action spectrum for growth delay induced in Escherichia coli B-r by far-ultraviolet radiation.
    J Bacteriol. 1969 May;98(2):677-82 PMID: 4891265
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.138.3.779-782.1979
Published
1979-06-00
Pages
779-82
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218104
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.138.3.779-782.1979 PMC Full Text View journal details
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