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PMID: 378970 Published · ppublish English Journal Article

Isolation and partial characterization of protein E, a major protein found in certain Escherichia coli K-12 mutant strains: relationship to other outer membrane proteins.

Journal of bacteriology ·Vol. 139 ·No. 2 ·1979-08-00 ·Pages 418-23

Chai TJ, Foulds J

Abstract

Escherichia coli outer membrane protein E was purified, and its amino acid composition and N-terminal amino acid were determined. The purified protein was shown to be immunologically and electrophoretically identical to proteins Ic (U. Henning, W. Schmidmayr, and I. Hindennach, Mol. Gen. Genet. 154:293-298, 1977) and e (W. van Alphen, N. van Selm, and B. Lugtenberg, Mol. Gen. Genet. 159:75-83, 1978). Proteins E, e, and Ic were also immunologically related to E. coli outer membrane protein Ia. Lugtenberg and co-workers (B. Lugtenberg, R. van Boxtel, C. Verhoef, and W. van Alphen, FEBS Lett. 96:99-105, 1978) have shown that electrophoretically identical peptides were generated by cyanogen bromide treatment of proteins E, e, and Ic.

MeSH Terms
Amino Acids/analysis Antigens, Bacterial/analysis Bacterial Proteins/analysis,immunology,isolation & purification Escherichia coli/analysis,immunology Membrane Proteins/analysis,immunology,isolation & purification Mutation
Chemicals
Amino Acids Antigens, Bacterial Bacterial Proteins Membrane Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chai T J
Foulds J
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.139.2.418-423.1979
Published
1979-08-00
Pages
418-23
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216885
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.139.2.418-423.1979 PMC Full Text View journal details
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