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PMID: 3800883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of low-protein diet and starvation on the activity of branched-chain 2-oxo acid dehydrogenase kinase in rat liver and heart.

The Biochemical journal ·Vol. 237 ·No. 1 ·1986-07-01 ·Pages 285-8

Espinal J, Beggs M, Patel H, Randle PJ

Abstract

The activity of branched-chain 2-oxo acid dehydrogenase kinase was 3-fold greater in extracts of heart mitochondria than in extracts of liver mitochondria from rats fed on normal diet. Feeding rats on a 0%-casein diet for 10 days increased the activity of branched-chain kinase 4-fold in extracts of liver mitochondria and in branched-chain dehydrogenase complex purified from such extracts; starvation (48 h) was without effect. In extract of heart mitochondria, kinase activity was increased 2-fold by feeding on 0%-casein diet and 1.5-fold by 48 h of starvation.

MeSH Terms

3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) Animals Electrophoresis, Polyacrylamide Gel Ketone Oxidoreductases/metabolism Male Mitochondria, Heart/enzymology Mitochondria, Liver/enzymology Multienzyme Complexes/metabolism Phosphorylation Protein Deficiency/enzymology Protein Kinases/metabolism Rats Rats, Inbred Strains Starvation/enzymology

Chemicals

Multienzyme Complexes Ketone Oxidoreductases 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) Protein Kinases (3-methyl-2-oxobutanoate dehydrogenase (lipoamide)) kinase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Espinal J

Beggs M

Patel H

Randle P J

References (12)

12 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2370285

Published

1986-07-01

Pages

285-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1146979

Subset

External Links

PubMed source DOI: 10.1042/bj2370285 PMC Full Text View journal details

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Effects of low-protein diet and starvation on the activity of branched-chain 2-oxo acid dehydrogenase kinase in rat liver and heart.

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