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PMID: 3805273 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Myocardial metabolism of free fatty acids. Studies with 14C-labeled substrates in humans.

The Journal of clinical investigation ·Vol. 79 ·No. 2 ·1987-02-00 ·Pages 359-66

Wisneski JA, Gertz EW, Neese RA, Mayr M

Abstract

Free fatty acids are considered to be the major energy source for the myocardium. To investigate the metabolic fate of this substrate in humans, 24 subjects underwent coronary sinus and arterial catheterization. 13 subjects were healthy volunteers and 11 subjects had symptoms of ischemic heart disease. [1-14C]oleate or [1-14C]palmitate bound to albumin was infused at a constant rate of 25 microCi/h. Oxidation was determined by measuring the 14CO2 production. The data demonstrated that a high percentage (84 +/- 17%) of the palmitate and oleate extracted by the myocardium underwent rapid oxidation. A highly significant correlation was present between the arterial level and the amount oxidized (r = 0.82, P less than 0.001 for palmitate; r = 0.77, P less than 0.001 for oleate). The isotope extraction ratio was greater than the chemical extraction ratio. This difference of 6 +/- 2 nmol/ml of blood in the young normal subjects was significantly less than the 12 +/- 4 nmol/ml observed in the ischemic heart disease patients (P less than 0.001).

MeSH Terms
Adolescent Adult Carbon Radioisotopes Fatty Acids, Nonesterified/blood,metabolism Humans Kinetics Male Myocardium/metabolism Oleic Acid Oleic Acids/metabolism Oxidation-Reduction Palmitic Acid Palmitic Acids/metabolism
Chemicals
Carbon Radioisotopes Fatty Acids, Nonesterified Oleic Acids Palmitic Acids Oleic Acid Palmitic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wisneski J A
Gertz E W
Neese R A
Mayr M
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49 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112820
Published
1987-02-00
Pages
359-66
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC424073
Subset
IM
Grants
NHLBI NIH HHS · HL-25625 · United States
External Links
PubMed source DOI: 10.1172/JCI112820 PMC Full Text View journal details
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