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PMID: 381197 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Surface protein of a Streptococcus agalactiae isolate.

Infection and immunity ·Vol. 24 ·No. 3 ·1979-06-00 ·Pages 637-41

de Cueninck BJ

Abstract

A Streptococcus agalactiae isolate of bovine origin was cultured in broth; log-phase cells were washed and radioiodinated and subsequently extracted at low pH in the presence of a nonionic detergent. A protein antigen was purified from concentrated extract by ultracentrifugation, gel filtration, and ion-exchange chromatography. The molecular weight of the protein was estimated at 31,800. The agglutinogenic character of the protein indicated its localization at the cell surface.

MeSH Terms
Antigens, Bacterial/isolation & purification Bacterial Proteins/immunology,isolation & purification Membrane Proteins/immunology,isolation & purification Molecular Weight Streptococcus agalactiae/analysis,immunology
Chemicals
Antigens, Bacterial Bacterial Proteins Membrane Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
de Cueninck B J
References (19)
19 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.24.3.637-641.1979
Published
1979-06-00
Pages
637-41
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414353
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.24.3.637-641.1979 PMC Full Text View journal details
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