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PMID: 381684 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Amino acid and sugar transport in Escherichia coli (ColIb) during abortive infection by bacteriophage T5.

Journal of virology ·Vol. 30 ·No. 2 ·1979-05-00 ·Pages 421-30

Glenn J, Duckworth DH

Abstract

T5 bacteriophage cannot replicate in Escherichia coli containing the colicinogenic factor ColIb. We show that active transport of proline and glutamine begins to decline at about 10 min after infection, the same time at which macromolecular synthesis stops during abortive infection. Uptake of alpha-methylglucoside is stimulated, however, and this change is evident even by 5 min after infection. These changes in membrane function do not occur during infections that are productive because of mutations on the plasmid or phage. The results suggest that the abortive infection is caused by membrane depolarization.

MeSH Terms
Amino Acids/metabolism Biological Transport, Active Cell Membrane/metabolism Colicins Coliphages/growth & development Escherichia coli/metabolism Glucose/metabolism Glutamine/metabolism Methylglucosides/metabolism Methylglycosides/metabolism Proline/metabolism RNA, Viral/biosynthesis Viral Proteins/biosynthesis
Chemicals
Amino Acids Colicins Methylglucosides Methylglycosides RNA, Viral Viral Proteins Glutamine Proline Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Glenn J
Duckworth D H
References (37)
37 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.30.2.421-430.1979
Published
1979-05-00
Pages
421-30
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353345
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.30.2.421-430.1979 PMC Full Text View journal details
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