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PMID: 3821723 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells.

Molecular and cellular biology ·Vol. 7 ·No. 2 ·1987-02-00 ·Pages 569-77

Looney JE, Hamlin JL

Abstract

We isolated overlapping recombinant cosmids that represent the equivalent of two complete dihydrofolate reductase amplicon types from the methotrexate-resistant CHO cell line CHOC400. The type I amplicons are 260 kilobases long, are arranged in head-to-tail fashion, and represent 10 to 15% of the amplicons in the CHOC400 genome. The type II amplicons are 220 kilobases long, are arranged in head-to-head and tail-to-tail configurations, and constituted the majority of the remaining amplicons in CHOC400 cells. The type II amplicon sequences are represented entirely within the type I unit. These are the first complete amplicons to be cloned from a mammalian cell line.

MeSH Terms
Animals Cell Line Chromosome Mapping Cloning, Molecular Cricetinae Drug Resistance Gene Amplification Genes Methotrexate/pharmacology Replicon Tetrahydrofolate Dehydrogenase/genetics
Chemicals
Tetrahydrofolate Dehydrogenase Methotrexate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Looney J E
Hamlin J L
References (30)
30 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.2.569-577.1987
Published
1987-02-00
Pages
569-77
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365110
Subset
IM
Grants
NIGMS NIH HHS · GM-09742 · United States
NIGMS NIH HHS · GM-26108 · United States
NIGMS NIH HHS · GM-32481 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.2.569-577.1987 PMC Full Text View journal details
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