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PMID: 3822769 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Measurement by Quin2 of changes of the intracellular calcium concentration in strips of the rabbit ear artery and of the guinea-pig ileum.

Pflugers Archiv : European journal of physiology ·Vol. 408 ·No. 1 ·1987-01-00 ·Pages 32-7

Himpens B, Casteels R

Abstract

Ca2+ transient and force development were investigated in smooth muscle strips of the rabbit ear artery and the longitudinal layer of the guinea-pig ileum by using the fluorescent indicator Quin2. Agonists only transiently increased the fluorescence intensity despite the enhanced contraction while excess potassium resulted in a maintained light signal. In Ca2+ free solutions the release by an agonist of Ca2+ from an intracellular store can be demonstrated. These observations illustrate the usefulness of the Ca2+ indicator Quin2 in the study of the excitation-contraction coupling in smooth muscle under various conditions.

MeSH Terms
Aminoquinolines/metabolism Animals Arteries/metabolism Calcium/metabolism Female Guinea Pigs Ileum/metabolism In Vitro Techniques Male Muscle Contraction/drug effects Muscle, Smooth/metabolism Muscle, Smooth, Vascular/metabolism Potassium/pharmacology Rabbits
Chemicals
Aminoquinolines Quin2 Potassium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Himpens B
Casteels R
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26 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00581837
Published
1987-01-00
Pages
32-7
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00581837 View journal details
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