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PMID: 3827824 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Further evidence for the involvement of a phosphoprotein in the respiratory burst oxidase of human neutrophils.

The Biochemical journal ·Vol. 239 ·No. 3 ·1986-11-01 ·Pages 723-31

Heyworth PG, Segal AW

Abstract

Phosphorylation of a 47 kDa protein in human neutrophils is induced by phorbol 12-myristate 13-acetate (PMA), opsonized latex beads, fMet-Leu-Phe, calcium ionophore A23187 and fluoride. All of these stimuli activate the specialized microbicidal respiratory burst of neutrophils, and in each case the kinetics of activation correspond with the kinetics of phosphorylation of the 47 kDa protein. Trifluoperazine (50 microM) and chlorpromazine (100 microM), inhibitors of calmodulin and protein kinase C, abolish the increase in oxygen consumption and selectively prevent phosphorylation of the 47 kDa protein after PMA stimulation. Treatment of neutrophils with pertussis toxin totally inhibits both superoxide production and phosphorylation of this protein in response to fMet-Leu-Phe, but not in response to PMA, indicating that a GTP-binding protein modulates the fMet-Leu-Phe receptor signal. Phosphorylation of the 47 kDa protein, a phenomenon absent from the neutrophils of subjects with autosomal recessive chronic granulomatous disease, which lack the respiratory burst, appears to be the common trigger for activation of the burst in normal neutrophils.

MeSH Terms
Autoradiography Blood Proteins/metabolism Electrophoresis, Polyacrylamide Gel Fluorides/pharmacology Humans NADH, NADPH Oxidoreductases/antagonists & inhibitors,blood NADPH Oxidases Neutrophils/drug effects,enzymology Pertussis Toxin Phosphoproteins/metabolism Phosphorylation Tetradecanoylphorbol Acetate/pharmacology Virulence Factors, Bordetella/pharmacology
Chemicals
Blood Proteins Phosphoproteins Virulence Factors, Bordetella NADH, NADPH Oxidoreductases NADPH Oxidases superoxide-forming enzyme Pertussis Toxin Tetradecanoylphorbol Acetate Fluorides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heyworth P G
Segal A W
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32 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2390723
Published
1986-11-01
Pages
723-31
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147346
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2390723 PMC Full Text View journal details
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