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PMID: 3839317 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of a gene sequence regulated by nerve growth factor.

Science (New York, N.Y.) ·Vol. 229 ·No. 4711 ·1985-07-26 ·Pages 393-5

Levi A, Eldridge JD, Paterson BM

Abstract

Nerve growth factor (NGF) is essential for the development and differentiation of sympathetic or sensory neurons. A complementary DNA was cloned that corresponds to a gene sequence induced more than 50-fold in a cultured target cell line of pheochromocytoma cells (PC12 cells) 5 hours after the addition of NGF. The induced messenger RNA encodes a 90,000-dalton polypeptide that may represent one of the primary events in NGF-induced differentiation of neurons.

MeSH Terms

Actins/genetics Adrenal Gland Neoplasms/genetics Animals Base Sequence Cell Line Chickens Cloning, Molecular DNA/genetics Gene Expression Regulation Genes Nerve Growth Factors/physiology Nucleic Acid Hybridization Pheochromocytoma/genetics RNA, Messenger/genetics Rabbits Rats

Chemicals

Actins Nerve Growth Factors RNA, Messenger DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Levi A

Eldridge J D

Paterson B M

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.3839317

Published

1985-07-26

Pages

393-5

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.3839317 View journal details

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Molecular cloning of a gene sequence regulated by nerve growth factor.

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