Home LiteratureArticle Details
PMID: 3840589 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of eukaryotic protein synthesis initiation factor genes: isolation and characterization of cDNA clones encoding factor eIF-4A.

Nucleic acids research ·Vol. 13 ·No. 19 ·1985-10-11 ·Pages 6867-80

Nielsen PJ, McMaster GK, Trachsel H

Abstract

Monoclonal antibodies directed against rabbit reticulocyte protein synthesis initiation factor 4A (eIF-4A) were used to isolate mouse cDNA clones expressing eIF-4A protein sequences in E. coli. The identity of cDNA clones encoding eIF-4A sequences was confirmed by hybrid-selected translation and peptide mapping of the translation product. Analysis of the mRNA coding for eIF-4A from mouse liver and HeLa cells by Northern hybridization revealed two discrete mRNA species of approximately 2000 and 1600 nucleotides in length. The existence of two mRNAs in mouse and HeLa cells encoding eIF-4A was confirmed by cDNA sequencing.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics Eukaryotic Initiation Factor-4A Humans Mice Peptide Fragments/analysis Peptide Initiation Factors/genetics,immunology Protein Biosynthesis RNA, Messenger/genetics Rabbits
Chemicals
Peptide Fragments Peptide Initiation Factors RNA, Messenger DNA Eukaryotic Initiation Factor-4A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nielsen P J
McMaster G K
Trachsel H
References (30)
30 references, click to expand
  1. Processing of adenovirus 2-induced proteins.
    J Virol. 1973 Aug;12(2):241-52 PMID: 4747985
  2. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  3. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  4. Purification and characterization of initiation factors IF-E4 and IF-E6 from rabbit reticulocytes.
    J Biol Chem. 1977 Aug 25;252(16):5798-803 PMID: 885883
  5. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  6. Initiation of mammalian protein synthesis. II. The assembly of the initiation complex with purified initiation factors.
    J Mol Biol. 1977 Nov;116(4):755-67 PMID: 592399
  7. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  8. Synthesis of full length cDNAs from four partially purified oviduct mRNAs.
    J Biol Chem. 1978 Apr 10;253(7):2471-82 PMID: 632280
  9. Post-transcriptional and translational controls of gene expression in eukaryotes.
    Annu Rev Biochem. 1978;47:1079-126 PMID: 354491
  10. The molecular mechanism of hemoglobin synthesis and its regulation in the reticulocyte.
    CRC Crit Rev Biochem. 1978;5(4):261-90 PMID: 363353
  11. Purification of seven protein synthesis initiation factors from Krebs II ascites cells.
    Biochim Biophys Acta. 1979 Feb 27;561(2):484-90 PMID: 427168
  12. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
    Anal Biochem. 1979 Sep 15;98(1):132-5 PMID: 543547
  13. Tissue-specific expression of mouse alpha-amylase genes.
    J Mol Biol. 1980 Sep 5;142(1):93-116 PMID: 6159478
  14. Characterization of plasma membrane proteins identified by monoclonal antibodies.
    J Biol Chem. 1981 Jan 25;256(2):664-71 PMID: 7451466
  15. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
    Anal Biochem. 1980 Jul 1;105(2):361-3 PMID: 6161559
  16. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
    Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 PMID: 7301588
  17. Characterization of eukaryotic initiation factor 4A, a protein involved in ATP-dependent binding of globin mRNA.
    J Biol Chem. 1982 May 10;257(9):5246-52 PMID: 7068683
  18. Initiation factors in protein biosynthesis.
    Annu Rev Biochem. 1982;51:869-900 PMID: 7051967
  19. Immunochemical characterization of mammalian protein synthesis initiation factors.
    Biochemistry. 1982 Aug 31;21(18):4206-12 PMID: 6751385
  20. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  21. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  22. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  23. Unusual requirements for optimum translation of polio viral RNA in vitro.
    J Biol Chem. 1983 Jun 10;258(11):7195-9 PMID: 6304083
  24. Identification and quantitation of levels of protein synthesis initiation factors in crude HeLa cell lysates by two-dimensional polyacrylamide gel electrophoresis.
    J Biol Chem. 1983 Jun 10;258(11):7228-35 PMID: 6853516
  25. New initiation factor activity required for globin mRNA translation.
    J Biol Chem. 1983 May 10;258(9):5804-10 PMID: 6853548
  26. Methods utilizing cell-free protein-synthesizing systems for the identification of recombinant DNA molecules.
    Methods Enzymol. 1983;101:650-74 PMID: 6577259
  27. Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
    Methods Enzymol. 1983;100:255-66 PMID: 6194404
  28. Yeast RNA polymerase II genes: isolation with antibody probes.
    Science. 1983 Nov 18;222(4625):778-82 PMID: 6356359
  29. Polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions.
    Nucleic Acids Res. 1983 Nov 25;11(22):7853-71 PMID: 6359064
  30. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.19.6867
Published
1985-10-11
Pages
6867-80
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322010
Subset
IM
Databases
GENBANK
X03039, X03040
External Links
PubMed source DOI: 10.1093/nar/13.19.6867 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2