Isolation and characterization of a cytolysin produced by Vibrio cholerae serogroup non-O1.

Canadian journal of microbiology ·Vol. 31 ·No. 8 ·1985-08-00 ·Pages 711-20

McCardell BA, Madden JM, Shah DB

Abstract

A thermolabile toxin (molecular weight, 52 711; isoelectric point, 8.65) produced by a clinical isolate of Vibrio cholerae serogroup non-O1 was cytotoxic for Y-1 mouse adrenal cells and Chinese hamster ovary cells. The toxin lysed rabbit red blood cells and produced a hemorrhagic zone in rabbit skin. When injected intravenously into adult mice, the cytolysin was rapidly lethal and caused fluid accumulation in both 5- and 18-h rabbit ileal loops. Strains of V. cholerae that produced cytolysin but no cholerae enterotoxin were able to cause fluid accumulation in rabbit intestinal loops.

MeSH Terms

Animals Bacterial Toxins/immunology Cell Line Cell Survival Chromatography, Gel Cricetinae Cricetulus Cytotoxins/immunology,isolation & purification,toxicity Enzyme-Linked Immunosorbent Assay Female Hemolysis Ileum Immune Sera Intestinal Mucosa/metabolism Isoelectric Point Lethal Dose 50 Male Mice Molecular Weight Neutralization Tests Rabbits Shiga Toxins Time Factors Vibrio cholerae/metabolism

Chemicals

Bacterial Toxins Cytotoxins Immune Sera Shiga Toxins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

McCardell B A

Madden J M

Shah D B

Article Info

Journal

Canadian journal of microbiology

Abbr.

Can J Microbiol

ISSN

0008-4166

DOI

10.1139/m85-135

Published

1985-08-00

Pages

711-20

Language

English

Region

Canada

NLM ID

0372707

Subset

External Links

PubMed source DOI: 10.1139/m85-135 View journal details

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Isolation and characterization of a cytolysin produced by Vibrio cholerae serogroup non-O1.

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