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PMID: 38463578 Published · epublish English Journal Article

CDCA8, a mitosis-related gene, as a prospective pan-cancer biomarker: implications for survival prognosis and oncogenic immunology.

American journal of translational research ·Vol. 16 ·No. 2 ·2024-00-00 ·Pages 432-445

Hu H, Umair M, Khan SA, Sani AI, Iqbal S, Khalid F, Sultan R, Abdel-Maksoud MA, Mubarak A, Dawoud TM, Malik A, Saleh IA, Al Amri AA, Algarzae NK, Kodous AS, Hameed Y

Abstract

Human cell division cycle-associated protein 8 (CDCA8), a critical regulator of mitosis, has been identified as a prospective prognostic biomarker in several cancer types, including breast, colon, and lung cancers. This study analyzed the diagnostic/prognostic potential and clinical implications of CDCA8 across diverse cancers. Bioinformatics and molecular experiments. Analyzing TCGA data via TIMER2 and GEPIA2 databases revealed significant up-regulation of CDCA8 in 23 cancer types compared to normal tissues. Prognostically, elevated CDCA8 expression correlated with poorer overall survival in KIRC, LUAD, and SKCM, emphasizing its potential as a prognostic marker. UALCAN analysis demonstrated CDCA8 up-regulation based on clinical variables, such as cancer stage, race, and gender, in these cancers. Epigenetic exploration indicated reduced CDCA8 promoter methylation levels in Kidney Renal Clear Cell Carcinoma (KIRC), Lung Adenocarcinoma (LUAD), and Skin Cutaneous Melanoma (SKCM) tissues compared to normal controls. Promoter methylation and mutational analyses showcased a hypomethylation and low mutation rate for CDCA8 in these cancers. Correlation analysis revealed positive associations between CDCA8 expression and infiltrating immune cells, particularly CD8+ and CD4+ T cells. Protein-protein interaction (PPI) network analysis unveiled key interacting proteins, while gene enrichment analysis highlighted their involvement in crucial cellular processes and pathways. Additionally, exploration of CDCA8-associated drugs through DrugBank presented potential therapeutic options for KIRC, LUAD, and SKCM. In vitro validation using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) confirmed elevated CDCA8 expression in LUAD cell lines (A549 and H1299) compared to control cell lines (Beas-2B and NL-20). This study provides concise insights into CDCA8's multifaceted role in KIRC, LUAD, and SKCM, covering expression patterns, diagnostic and prognostic relevance, epigenetic regulation, mutational landscape, immune infiltration, and therapeutic implications.

Keywords
CDCA8 biomarker pan-cancer prognosis treatment
Authors & Affiliations
16 authors, click to expand affiliations / ORCID
Hu Hanjie
Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021, China.
Umair Muhammad
Department of Physiology, Gomal Medical College, MTI Dera Ismail Khan, Pakistan.
Khan Sikandar Ali
Department of Biochemistry Khyber Girls Medical College Peshawar, Pakistan.
Sani Aliya Irshad
Department of Biochemistry, Ziauddin Medical College Karachi 74700, Pakistan.
Iqbal Sahar
Department of Pathology, Azra Naheed Medical College Lahore 54000, Pakistan.
Khalid Fatima
Department of Pathology, Al Aleem Medical College Lahore, Pakistan.
Sultan Rizwana
Department of Pathology, Faculty of Veterinary and Animal Sciences, Cholistan University of Veterinary and Animal Sciences Bahawalpur, Pakistan.
Abdel-Maksoud Mostafa A
Department of Botany and Microbiology, College of Science, King Saud University P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Mubarak Ayman
Department of Botany and Microbiology, College of Science, King Saud University P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Dawoud Turki M
Department of Botany and Microbiology, College of Science, King Saud University P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Malik Abdul
Department of Pharmaceutics, College of Pharmacy, King Saud University Saudi Arabia.
Saleh Ibrahim A
Faculty of Science, Zarqa University Zarqa 13110, Jordan.
Al Amri Abdul Aziz
Biochemistry Department, College of Science, King Saud University P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Algarzae Norah Khaled
Department of Physiology, College of Medicine, King Saud University Riyadh 11149, Saudi Arabia.
Kodous Ahmad S
Radiation Biology Department, National Center for Radiation Research & Technology (NCRRT), Egyptian Atomic-Energy Authority (EAEA) Egypt. | Department of Molecular Oncology, Cancer Institute (WIA) 38, Sardar Patel Road, Chennai, P.O. Box 600036, Tamilnadu, India.
Hameed Yasir
Department of Biotechnology, Institute of Biochemistry Biotechnology and Bioinformatics, The Islamia University of Bahawalpur Bahawalpur 63100, Pakistan.
Conflict of Interest

None.

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Article Info
Journal
American journal of translational research
Abbr.
Am J Transl Res
ISSN
1943-8141
Published
2024-00-00
Epub
2024-00-15
Pages
432-445
Language
English
Region
United States
NLM ID
101493030
PMCID
PMC10918119
External Links
PubMed source PMC Full Text View journal details
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