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PMID: 3856844 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and nucleotide sequence determination of the Clostridium pasteurianum ferredoxin gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 6 ·1985-03-00 ·Pages 1653-7

Graves MC, Mullenbach GT, Rabinowitz JC

Abstract

We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain greater than 6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobase-pair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence.

MeSH Terms
Base Sequence Cloning, Molecular Clostridium/genetics Codon/genetics DNA, Bacterial/genetics Ferredoxins/genetics Genes, Bacterial Operon Protein Biosynthesis Transcription, Genetic
Chemicals
Codon DNA, Bacterial Ferredoxins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Graves M C
Mullenbach G T
Rabinowitz J C
References (31)
31 references, click to expand
  1. Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium.
    Nucleic Acids Res. 1981 Jun 11;9(11):2577-88 PMID: 6269055
  2. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
  3. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  4. Structure of the Fe-S complex in a bacterial ferredoxin.
    Nature. 1972 Jan 7;235(5332):40-2 PMID: 4550392
  5. Translation of synthetic and endogenous messenger ribonucleic acid in vitro by ribosomes and polyribosomes from Clostridium pasteurianum.
    J Bacteriol. 1972 Dec;112(3):1057-69 PMID: 4565527
  6. Initiation of protein synthesis in vitro by a clostridial system. I. Specificity in the translation of natural messenger ribonucleic acids.
    J Biol Chem. 1973 May 10;248(9):3209-15 PMID: 4573349
  7. Preparation and properties of clostridial ferredoxins.
    Methods Enzymol. 1972;24:431-46 PMID: 4670202
  8. Ribsome and messenger specificity in protein synthesis by bacteria.
    Biochem Biophys Res Commun. 1974 May 7;58(1):92-8 PMID: 4208644
  9. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  10. Utilization of promoter and terminator sites on bacteriophage T7 DNA by RNA polymerases from a variety of bacterial orders.
    Cell. 1979 Jan;16(1):97-109 PMID: 421272
  11. Filter replicas and permanent collections of recombinant DNA plasmids.
    Nucleic Acids Res. 1979 Dec 20;7(8):2115-36 PMID: 118435
  12. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  13. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  14. Clostridial apoferredoxin messenger ribonucleic acid. Assay and partial purification.
    Biochim Biophys Acta. 1980 Jul 29;608(2):301-14 PMID: 7397188
  15. RNA polymerase from Clostridium acidi-urici. Characterization of a naturally occurring rifampicin-resistant bacterial enzyme.
    J Biol Chem. 1981 May 25;256(10):5153-61 PMID: 7228873
  16. Cascades of Sigma factors.
    Cell. 1981 Sep;25(3):582-4 PMID: 6793235
  17. Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene.
    J Biol Chem. 1981 Nov 10;256(21):11283-91 PMID: 6793593
  18. Nucleotide sequences of transcription and translation initiation regions in Bacillus phage phi 29 early genes.
    J Biol Chem. 1982 Jan 25;257(2):1053-62 PMID: 6274853
  19. Characterization of translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
  20. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis.
    Mol Gen Genet. 1982;186(3):339-46 PMID: 6181373
  21. Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli.
    Nucleic Acids Res. 1983 May 25;11(10):3065-76 PMID: 6304620
  22. Cloning and expression of the hydrogenase gene from Clostridium butyricum in Escherichia coli.
    FEBS Lett. 1983 Jul 11;158(1):119-22 PMID: 6345200
  23. Cloning of the Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase gene in Escherichia coli. Nucleotide sequence determination and properties of the plasmid-encoded enzyme.
    J Biol Chem. 1983 Sep 10;258(17):10586-93 PMID: 6411717
  24. Hybridization of nucleic acids directly in agarose gels.
    Anal Biochem. 1983 Jun;131(2):365-72 PMID: 6193737
  25. Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis.
    Nucleic Acids Res. 1983 Nov 25;11(22):7911-25 PMID: 6316278
  26. Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7461-5 PMID: 6369317
  27. Genome complexity of methanogenic bacteria.
    J Bacteriol. 1984 May;158(2):628-31 PMID: 6725206
  28. The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.
    Nucleic Acids Res. 1984 Jul 11;12(13):5355-67 PMID: 6087283
  29. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.
    Biochim Biophys Acta. 1963 Aug 20;72:619-29 PMID: 14071565
  30. THE C- AND N-TERMINAL AMINO ACID SEQUENCES OF CLOSTRIDIUM PASTEURIANUM FERREDOXIN.
    Arch Biochem Biophys. 1964 Jun;105:570-4 PMID: 14236643
  31. DEOXYRIBONUCLEIC ACID BASE COMPOSITION OF CLOSTRIDIAL SPECIES.
    J Bacteriol. 1965 May;89:1438-9 PMID: 14293020
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.6.1653
Published
1985-03-00
Pages
1653-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397330
Subset
IM
Grants
NIAID NIH HHS · AI06712 · United States
NIADDK NIH HHS · AM2109 · United States
Databases
GENBANK
M11214, M13633, M13682
External Links
PubMed source DOI: 10.1073/pnas.82.6.1653 PMC Full Text View journal details
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