Purification and characterization of two cartilage-inducing factors from bovine demineralized bone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 8 ·1985-04-00 ·Pages 2267-71

Seyedin SM, Thomas TC, Thompson AY, Rosen DM, Piez KA

Abstract

Two naturally occurring peptides that induce chondrogenesis in culture have been purified to apparent homogeneity. These cartilage-inducing factors (CIF-A and CIF-B) were isolated from bovine demineralized bone by dissociative extraction, gel filtration, cation-exchange chromatography, and reversed-phase HPLC. CIF-A and CIF-B at concentrations of 1-10 ng/ml each induce embryonic rat mesenchymal cells in culture to assume a cartilage morphology and synthesize cartilage-specific proteoglycan and type II collagen. The amino acid compositions of CIF-A and CIF-B are similar but not identical. Both factors have an apparent Mr of 26,000, as determined by NaDodSO4/PAGE. In the presence of 2-mercaptoethanol, both are converted to species of about one-half that Mr, indicating that they are dimers of identical or very similar chains.

MeSH Terms

Animals Bone and Bones/analysis Cartilage/growth & development Cattle Cells, Cultured Collagen/biosynthesis Growth Substances/isolation & purification Proteins/isolation & purification,pharmacology Proteoglycans/biosynthesis Rats

Chemicals

Growth Substances Proteins Proteoglycans Collagen

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Seyedin S M

Thomas T C

Thompson A Y

Rosen D M

Piez K A

References (19)

19 references, click to expand

Bone: formation by autoinduction.
Science. 1965 Nov 12;150(3698):893-9 PMID: 5319761
Osteogenic induction by osteosarcoma and normal bone in mice.
J Bone Joint Surg Am. 1968 Mar;50(2):311-25 PMID: 4868305
High resolution acrylamide gel electrophoresis of histones.
Arch Biochem Biophys. 1969 Mar;130(1):337-46 PMID: 5778650
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Isolation and characterization of a collagen from chick cartilage containing three identical alpha chains.
Biochemistry. 1971 Apr 27;10(9):1652-9 PMID: 4325605
Biochemical sequences in the transformation of normal fibroblasts in adolescent rats.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1601-5 PMID: 4504376
Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
Bone morphogenesis in implants of residues of radioisotope labelled bone matrix.
Calcif Tissue Res. 1974;15(4):269-86 PMID: 4441968
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
Solubilized bone morphogenetic protein (BMP) from mouse osteosarcoma and rat demineralized bone matrix.
Clin Orthop Relat Res. 1980 May;(148):281-90 PMID: 6929747
Analysis of cartilage differentiation from skeletal muscle grown on bone matrix. II. Chondroitin sulfate synthesis and reaction to exogenous glycosaminoglycans.
Dev Biol. 1980 Aug;78(2):332-51 PMID: 6773835
An enzyme-linked immunoassay for the cartilage proteoglycan.
Arch Biochem Biophys. 1981 Apr 1;207(2):399-406 PMID: 7247411
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
Anal Biochem. 1981 Nov 1;117(2):307-10 PMID: 6172996
Dissociative extraction and reconstitution of extracellular matrix components involved in local bone differentiation.
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7599-603 PMID: 6950401
Cell biology and biochemistry of endochondral bone development.
Coll Relat Res. 1981 Feb;1(2):209-26 PMID: 7049548
Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels.
Cell. 1982 Aug;30(1):215-24 PMID: 7127471
In vitro induction of cartilage-specific macromolecules by a bone extract.
J Cell Biol. 1983 Dec;97(6):1950-3 PMID: 6643582
Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography.
Proc Natl Acad Sci U S A. 1984 Jan;81(2):371-5 PMID: 6320184
Osteogenic induction across millipore filters in vivo.
Science. 1961 Jun 30;133(3470):2065-7 PMID: 13706516

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.8.2267

Published

1985-04-00

Pages

2267-71

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC397538

Subset

External Links

PubMed source DOI: 10.1073/pnas.82.8.2267 PMC Full Text View journal details

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Purification and characterization of two cartilage-inducing factors from bovine demineralized bone.

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