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PMID: 3857590 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Synthesis and secretion of proteins resembling platelet-derived growth factor by human glioblastoma and fibrosarcoma cells in culture.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 8 ·1985-04-00 ·Pages 2404-8

Pantazis P, Pelicci PG, Dalla-Favera R, Antoniades HN

Abstract

Immunoprecipitation of proteins extracted from metabolically labeled human glioblastoma and fibrosarcoma cells with antiserum to platelet-derived growth factor (PDGF) showed that these cells express and secrete proteins that are recognized specifically by the antiserum. The molecular masses of immunoprecipitated proteins in the lysates of the malignant cells ranged from 16 kDa to 140 kDa. Both cell lines secreted a 31-kDa polypeptide with structural, immunological, and biological properties similar to those of human PDGF. These cell lines were shown to synthesize a 4.4-kb mRNA that contained sequences from all the six currently identified exons of the human c-sis gene. These data suggest that the PDGF-like proteins in the two mesenchyme-derived transformed cells are encoded at least in part by the c-sis locus.

MeSH Terms
Cell Line Fibrosarcoma/genetics,metabolism Glioma/genetics,metabolism Humans Oncogenes Platelet-Derived Growth Factor/biosynthesis,genetics RNA, Messenger/genetics
Chemicals
Platelet-Derived Growth Factor RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pantazis P
Pelicci P G
Dalla-Favera R
Antoniades H N
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.8.2404
Published
1985-04-00
Pages
2404-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397566
Subset
IM
Grants
NCI NIH HHS · P30 CA010815 · United States
NCI NIH HHS · CA-30101 · United States
NCI NIH HHS · CA371650 · United States
NHLBI NIH HHS · HL-29583 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.8.2404 PMC Full Text View journal details
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