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PMID: 3858830 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Gene organization of DC and DX subregions of the human major histocompatibility complex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 10 ·1985-05-00 ·Pages 3410-4

Okada K, Boss JM, Prentice H, Spies T, Mengler R, Auffray C, Lillie J, Grossberger D, Strominger JL

Abstract

The DC and DX subregions of the human major histocompatibility complex (MHC) have been cloned from a cosmid library made from a human B-cell line, Priess. The DC subregion, 48 kilobases, includes the DC alpha and DC beta genes. A second DC-like region, the DX subregion, 35 kilobases, contains the DX alpha gene and a newly found beta gene termed DX beta. Since the DC and DX genes are highly homologous in nucleotide sequence, gene size, exon-intron organization, and direction of transcription, the DC and DX subregions were presumably generated by duplication of an ancestral alpha-beta gene pair. Nucleotide sequencing indicates that all four genes have intact coding sequences and promoter regions. Homology between the upstream promoter sequences of these four genes and seven other class II genes at nucleotides -69 to -78 and -98 to -110 highlights these previously described conserved elements. Moreover, a striking conservation of flanking alpha-gene-specific and beta-gene-specific sequences has been observed. Comparison of Southern blots of Priess DNA with DC alpha and DC beta cDNA probes with isolated cosmid clones showed that (i) the human chromosome encodes only two DC alpha-related and two DC beta-related genes, namely, DC alpha, DX alpha, DC beta, and DX beta, and (ii) the DC and DX subregions are homozygous in Priess cells.

MeSH Terms
Chromosome Mapping Cloning, Molecular Genes Histocompatibility Antigens Class II/genetics Humans Macromolecular Substances Major Histocompatibility Complex Peptides/genetics Promoter Regions, Genetic Protein Sorting Signals
Chemicals
Histocompatibility Antigens Class II Macromolecular Substances Peptides Protein Sorting Signals
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Okada K
Boss J M
Prentice H
Spies T
Mengler R
Auffray C
Lillie J
Grossberger D
Strominger J L
References (34)
34 references, click to expand
  1. Polymorphism and complexity of the human DC and murine I-A alpha chain genes.
    EMBO J. 1983;2(1):121-4 PMID: 11894898
  2. Isotypic and allotypic variation of human class II histocompatibility antigen alpha-chain genes.
    Nature. 1984 Mar 22-28;308(5957):327-33 PMID: 6584734
  3. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  4. HLA-DR light chain has a polymorphic N-terminal region and a conserved immunoglobulin-like C-terminal region.
    Nature. 1982 Jun 24;297(5868):694-7 PMID: 6953314
  5. Complete amino acid sequence of an HLA-DR antigen-like beta chain as predicted from the nucleotide sequence: similarities with immunoglobulins and HLA-A, -B, and -C antigens.
    Proc Natl Acad Sci U S A. 1982 Jun;79(12):3687-91 PMID: 6954511
  6. Alpha and beta chains of SB and DR antigens are structurally distinct.
    J Exp Med. 1982 Nov 1;156(5):1557-62 PMID: 6182264
  7. Organization of the transcriptional unit of a human class II histocompatibility antigen: HLA-DR heavy chain.
    Nucleic Acids Res. 1983 Dec 20;11(24):8663-75 PMID: 6324094
  8. Class II HLA-DC beta-chain DNA restriction fragments differentiate among HLA-DR2 individuals in insulin-dependent diabetes and multiple sclerosis.
    Proc Natl Acad Sci U S A. 1984 Mar;81(6):1774-8 PMID: 6324215
  9. Both alpha and beta chains of HLA-DC class II histocompatibility antigens display extensive polymorphism in their amino-terminal domains.
    EMBO J. 1984 Feb;3(2):447-52 PMID: 6585297
  10. Six HLA-D region alpha-chain genes on human chromosome 6: polymorphisms and associations of DC alpha-related sequences with DR types.
    Proc Natl Acad Sci U S A. 1984 Jun;81(11):3461-5 PMID: 6328517
  11. Recombination within the HLA-D region. Correlation of molecular genotyping with functional data.
    J Exp Med. 1984 Jul 1;160(1):222-38 PMID: 6330268
  12. Immune interferon activates multiple class II major histocompatibility complex genes and the associated invariant chain gene in human endothelial cells and dermal fibroblasts.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4917-21 PMID: 6431411
  13. Linkage map of two HLA-SB beta and two HLA-SB alpha-related genes: an intron in one of the SB beta genes contains a processed pseudogene.
    Cell. 1984 Aug;38(1):241-9 PMID: 6088068
  14. Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5199-203 PMID: 6206493
  15. Structure and polymorphism of the HLA class II SB light chain genes.
    EMBO J. 1984 Dec 1;3(12):2985-93 PMID: 6098459
  16. Characterization of an HLA DR beta pseudogene.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1475-9 PMID: 3856276
  17. Genes of the major histocompatibility complex of the mouse.
    Annu Rev Immunol. 1983;1:529-68 PMID: 6152713
  18. Allelic polymorphism and complexity of the genes for HLA-DR beta-chains--direct analysis by DNA-DNA hybridization.
    Nature. 1982 Nov 25;300(5890):372-4 PMID: 6292729
  19. cDNA clone for the heavy chain of the human B cell alloantigen DC1: strong sequence homology to the HLA-DR heavy chain.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6337-41 PMID: 6815651
  20. The construction of cosmid libraries which can be used to transform eukaryotic cells.
    Nucleic Acids Res. 1982 Nov 11;10(21):6715-32 PMID: 6294612
  21. The murine E alpha immune response gene.
    Cell. 1983 Mar;32(3):745-54 PMID: 6403249
  22. Sequences related to HLA-DR alpha chain on human chromosome 6: restriction enzyme polymorphism detected with DC alpha chain probes.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1972-6 PMID: 6300884
  23. Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity.
    J Exp Med. 1983 May 1;157(5):1461-70 PMID: 6189938
  24. A minimum of four human class II alpha-chain genes are encoded in the HLA region of chromosome 6.
    Nature. 1983 Jul 14-20;304(5922):174-7 PMID: 6306475
  25. The complete nucleotide sequence of the I-E alpha d immune response gene.
    Nucleic Acids Res. 1983 Aug 11;11(15):5055-71 PMID: 6308570
  26. Structure of the murine immune response I-A beta locus: sequence of the I-A beta gene and an adjacent beta-chain second domain exon.
    Cell. 1983 Aug;34(1):179-88 PMID: 6411350
  27. Complete primary structures of the E beta chain and gene of the mouse major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5520-4 PMID: 6310581
  28. Genetic mapping of a human class II antigen beta-chain cDNA clone to the SB region of the HLA complex.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):6036-40 PMID: 6310612
  29. An HLA-DR5 homozygous cell line expresses two DS (I-A-like) molecules.
    J Exp Med. 1983 Oct 1;158(4):1374-9 PMID: 6604786
  30. Genes of the major histocompatibility complex in mouse and man.
    Science. 1983 Nov 18;222(4625):727-33 PMID: 6356354
  31. Exon-intron organization and complete nucleotide sequence of a human major histocompatibility antigen DC beta gene.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7313-7 PMID: 6316358
  32. The class II molecules of the human and murine major histocompatibility complex.
    Cell. 1984 Jan;36(1):1-13 PMID: 6198089
  33. Primary structural sequence polymorphism in the human class II MHC antigen 33.1.
    Immunogenetics. 1984;19(1):27-37 PMID: 6319275
  34. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.10.3410
Published
1985-05-00
Pages
3410-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397785
Subset
IM
Grants
NIADDK NIH HHS · AM-30241 · United States
Databases
GENBANK
M11136, M11137
External Links
PubMed source DOI: 10.1073/pnas.82.10.3410 PMC Full Text View journal details
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