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PMID: 3858871 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Type beta transforming growth factor/growth inhibitor stimulates entry of monolayer cultures of AKR-2B cells into S phase after a prolonged prereplicative interval.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 12 ·1985-06-00 ·Pages 4147-51

Shipley GD, Tucker RF, Moses HL

Abstract

Type beta transforming growth factor/growth inhibitor (TGF-beta/GI) is demonstrated to be a potent stimulator of DNA synthesis in AKR-2B mouse embryo cells with a prolonged (greater than 24 hr) prereplicative phase when compared with other growth factors (epidermal growth factor, platelet-derived growth factor, or fibroblast growth factor) that induce DNA synthesis 12-14 hr after stimulation. In addition, TGF-beta/GI inhibits the early peak of DNA synthesis produced by EGF and insulin before the later stimulatory effects of TGF-beta/GI become manifest. TGF-beta/GI induces a marked morphologic transformation in these cells prior to their entry into S phase. Like the other growth factors, TGF-beta/GI stimulates an early increase in the rate of protein synthesis in AKR-2B cells and its stimulatory effect on DNA synthesis is enhanced by insulin. The data show that this molecule is a growth factor for certain mesenchymal cells in monolayer culture but only after a prereplicative phase that is significantly longer than that of other growth factors.

MeSH Terms
Animals Cells, Cultured/cytology Culture Media DNA/biosynthesis DNA Replication/drug effects Humans Interphase/drug effects Mice Mice, Inbred AKR Mitosis/drug effects Peptides/pharmacology Protein Biosynthesis Time Factors Transforming Growth Factors
Chemicals
Culture Media Peptides Transforming Growth Factors DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shipley G D
Tucker R F
Moses H L
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.12.4147
Published
1985-06-00
Pages
4147-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397952
Subset
IM
Grants
NCI NIH HHS · CA 09441 · United States
NCI NIH HHS · CA 16816 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.12.4147 PMC Full Text View journal details
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