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PMID: 3863109 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 19 ·1985-10-00 ·Pages 6507-11

Esch F, Baird A, Ling N, Ueno N, Hill F, Denoroy L, Klepper R, Gospodarowicz D, Böhlen P, Guillemin R

Abstract

The two major mitogenic polypeptides for endothelial cells have been purified to homogeneity. The complete primary structure of bovine pituitary basic fibroblast growth factor (FGF) and the amino-terminal amino acid sequence of bovine brain acidic FGF have been established by gas-phase sequence analyses. Homogeneous preparations of these polypeptides are potent mitogens (basic FGF, ED50 approximately equal to 60 pg/ml; acidic FGF ED50 approximately equal to 6000 pg/ml) for many diverse cell types including capillary endothelial cells, vascular smooth muscle cells, and adrenocortical and granulosa cells; in vivo, basic FGF is a powerful angiogenic agent in the chick chorioallantoic membrane assay. The available protein sequence data demonstrate the existence of significant structural homology between the two polypeptides.

MeSH Terms
Amino Acid Sequence Animals Cattle Cell Division/drug effects Cells, Cultured Cysteine/analysis Fibroblast Growth Factors/analysis,isolation & purification,pharmacology Hydrogen-Ion Concentration Mesoderm Pituitary Gland/analysis Protein Conformation Structure-Activity Relationship
Chemicals
Fibroblast Growth Factors Cysteine
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Esch F
Baird A
Ling N
Ueno N
Hill F
Denoroy L
Klepper R
Gospodarowicz D
Böhlen P
Guillemin R
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.19.6507
Published
1985-10-00
Pages
6507-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390746
Subset
IM
Grants
NIADDK NIH HHS · AM18811 · United States
NICHD NIH HHS · HD09690 · United States
NHLBI NIH HHS · HL20197 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.19.6507 PMC Full Text View journal details
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