Home LiteratureArticle Details
PMID: 3874400 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Inositol trisphosphate modification of ion transport in rough endoplasmic reticulum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 13 ·1985-07-00 ·Pages 4433-7

Muallem S, Schoeffield M, Pandol S, Sachs G

Abstract

The ion transport properties of the rough endoplasmic reticulum (RER) from liver have been defined by using measurements of active and potential gradient-driven transport. The Ca2+ pump is shown to be electrogenic, and both ATP and potential difference is able to drive vanadate-inhibitable Ca2+ uptake into the RER. ATP-dependent Ca2+ transport into the RER depends on the presence of tetraethylammonium-sensitive cation conductance and a furosemide-inhibited cation/chloride cotransport pathway. Inositol trisphosphate does not affect either of the monovalent ion translocation systems but activates a Ca2+ conductance in the RER, allowing efflux of RER Ca2+ stores into the cytosol in exchange for K+ uptake.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Calcium/metabolism Cesium/pharmacology Chlorides/metabolism Electric Conductivity Endoplasmic Reticulum/metabolism Furosemide/pharmacology Inositol 1,4,5-Trisphosphate Inositol Phosphates/metabolism Liver/ultrastructure Membrane Potentials Potassium/metabolism Rats Rubidium/metabolism Sugar Phosphates/metabolism Tetraethylammonium Tetraethylammonium Compounds/pharmacology
Chemicals
Chlorides Inositol Phosphates Sugar Phosphates Tetraethylammonium Compounds Cesium Tetraethylammonium Furosemide Inositol 1,4,5-Trisphosphate Adenosine Triphosphate cesium chloride Rubidium Potassium Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Muallem S
Schoeffield M
Pandol S
Sachs G
References (14)
14 references, click to expand
  1. Experimentally observed effects of carriers on the electrical properties of bilayer membranes--equilibrium domain. With a contribution on the molecular basis of ion selectivity.
    Membranes. 1973;2:179-328 PMID: 4585227
  2. Isolation of microsomal subfractions by use of density gradients.
    Methods Enzymol. 1978;52:71-82 PMID: 672657
  3. Is the red cell calcium pump electrogenic?
    J Physiol. 1982 Jun;327:1-15 PMID: 6288923
  4. Anion-dependent cation transport in erythrocytes.
    Philos Trans R Soc Lond B Biol Sci. 1982 Dec 1;299(1097):483-95 PMID: 6130541
  5. Chemiosmotic ATPase mechanisms.
    Ann N Y Acad Sci. 1982;402:584-601 PMID: 6188405
  6. A simple and sensitive procedure for measuring isotope fluxes through ion-specific channels in heterogenous populations of membrane vesicles.
    J Biol Chem. 1983 Nov 10;258(21):13094-9 PMID: 6195158
  7. Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.
    Nature. 1983 Nov 3-9;306(5938):67-9 PMID: 6605482
  8. Intracellular free calcium concentrations in isolated pancreatic acini; effects of secretagogues.
    Biochem Biophys Res Commun. 1983 Nov 30;117(1):122-8 PMID: 6197969
  9. myo-Inositol 1,4,5-trisphosphate. A second messenger for the hormonal mobilization of intracellular Ca2+ in liver.
    J Biol Chem. 1984 Mar 10;259(5):3077-81 PMID: 6607924
  10. Calcium messenger system: an integrated view.
    Physiol Rev. 1984 Jul;64(3):938-84 PMID: 6330778
  11. Turnover of inositol phospholipids and signal transduction.
    Science. 1984 Sep 21;225(4668):1365-70 PMID: 6147898
  12. Characterization of calcium uptake into rough endoplasmic reticulum of rat pancreas.
    J Membr Biol. 1984;81(1):69-82 PMID: 6208363
  13. Effect of inositol-1,4,5-trisphosphate on isolated subcellular fractions of rat pancreas.
    J Membr Biol. 1984;81(3):241-53 PMID: 6334162
  14. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.13.4433
Published
1985-07-00
Pages
4433-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391115
Subset
IM
Grants
NIADDK NIH HHS · AM 17328 · United States
NIADDK NIH HHS · AM 33010 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.13.4433 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2