Isolation and partial characterization of two different heat-stable enterotoxins produced by bovine and porcine strains of enterotoxigenic Escherichia coli.

Infection and immunity ·Vol. 26 ·No. 1 ·1979-10-00 ·Pages 173-7

Kapitany RA, Forsyth GW, Scoot A, McKenzie SF, Worthington RW

Abstract

Heat-stable enterotoxins (ST-124 and ST-1261) have been isolated from two different enterotoxigenic Escherichia coli of bovine (124) and porcine (1261) origin. The enterotoxin preparations were isolated by ultrafiltration and ion-exchange chromatography and were both active in the suckling mouse test and pig ligated loop test in the nanogram range. The bovine (ST-124) enterotoxin was not stable to heating in its isolated form, and significant differences in amino acid composition were observed between the two enterotoxins. Although both toxins were active at similar levels in the suckling mouse and pig ligated loop tests, ST-124 lacked the ability to cause the profound secretory responses seen with ST-1261 in the weanling pig ligated loop.

MeSH Terms

Amino Acids/analysis Animals Bacterial Toxins/analysis,isolation & purification,pharmacology Cattle Enterotoxins/analysis,isolation & purification,pharmacology Escherichia coli/analysis Hot Temperature Swine

Chemicals

Amino Acids Bacterial Toxins Enterotoxins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Kapitany R A

Forsyth G W

Scoot A

McKenzie S F

Worthington R W

References (24)

24 references, click to expand

Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Characterization of a partially purified methanol-soluble heat-stable Escherichia coli enterotoxin in infant mice.
Infect Immun. 1978 Mar;19(3):779-84 PMID: 346484
Cholera toxin effects on fluid secretion, adenylate cyclase, and cyclic AMP in porcine small intestine.
Infect Immun. 1978 Aug;21(2):373-80 PMID: 80378
Nutrition and enterotoxin synthesis by enterotoxigenic strains of Escherichia coli: defined medium for production of heat-stable enterotoxin.
Infect Immun. 1977 Mar;15(3):781-8 PMID: 15947
A new spectrophotometric assay for protein in cell extracts.
Anal Biochem. 1977 Oct;82(2):362-71 PMID: 20815
The effect of cholera toxin and heat labile and heat stable Escherichia coli enterotoxin on cyclic AMP concentrations in small intestinal mucosa of pig and rabbit.
Can J Comp Med. 1978 Jul;42(3):327-31 PMID: 210913
Purification and chemical characterization of the heat-stable enterotoxin produced by porcine strains of enterotoxigenic Escherichia coli.
Infect Immun. 1978 Mar;19(3):1021-30 PMID: 346481
Effect of heat stable and heat labile Escherichia coli enterotoxins, cholera toxin and theophylline on unidirectional sodium and chloride fluxes in the proximal and distal jejunum of weanling swine.
Can J Comp Med. 1977 Jul;41(3):306-17 PMID: 332292
Assay of Escherichia coli enterotoxins by in vivo perfusion in the rat jejunum.
Infect Immun. 1976 Oct;14(4):1004-10 PMID: 791862
Stimulation of intestinal adenyl cyclase by Escherichia coli enterotoxin: comparison of strains from an infant and an adult with diarrhea.
J Infect Dis. 1974 Jan;129(1):1-9 PMID: 4148810
Stimulation of adenyl cyclase by Escherichia coli enterotoxin.
Nat New Biol. 1972 Apr 5;236(66):137-8 PMID: 4337121
Biochemical properties of Escherichia coli low-molecular-weight, heat-stable enterotoxin.
Infect Immun. 1974 Feb;9(2):342-7 PMID: 4593342
Plasmid-associated enterotoxin production in a strain of Escherichia coli isolated from humans.
Infect Immun. 1972 Apr;5(4):622-4 PMID: 4564682
Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu.
J Infect Dis. 1972 Apr;125(4):407-11 PMID: 4553083
Dialysis and ultrafiltration of heat-stable enterotoxin from Escherichia coli.
J Med Microbiol. 1972 Aug;5(3):337-43 PMID: 4560751
Gas-liquid chromatography of N-heptafluorobutyryl isobutyl esters of amino acids.
J Chromatogr. 1974 Oct 9;97(1):19-24 PMID: 4421841
Pathogenesis of experimental cholera: biologic ativities of purified procholeragen A.
J Immunol. 1966 Mar;96(3):440-9 PMID: 4956587
The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.
J Med Microbiol. 1970 Aug;3(3):387-401 PMID: 4919579
Development of resistance with age by swine intestine to effects of enteropathogenic Escherichia coli.
J Infect Dis. 1970 Sep;122(3):220-3 PMID: 4915514
A heat-labile enterotoxin from strains of Eschericha coli enteropathogenic for pigs.
J Infect Dis. 1969 Oct;120(4):419-26 PMID: 4898783
Studies on Escherichia coli enterotoxin.
J Pathol Bacteriol. 1967 Apr;93(2):531-43 PMID: 4861401
Observations by the ligated intestinal segment and oral inoculation methods on Escherichia coli infections in pigs, calves, lambs and rabbits.
J Pathol Bacteriol. 1967 Apr;93(2):499-529 PMID: 4861400
Heat-stable Escherichia coli enterotoxin production in vivo.
Infect Immun. 1975 Aug;12(2):240-4 PMID: 1097335
Rapid formation of amino acid isobutyl esters for gas chromatography.
J Chromatogr. 1975 Sep 3;111(2):413-5 PMID: 1159019

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.26.1.173-177.1979

Published

1979-10-00

Pages

173-7

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC414590

Subset

External Links

PubMed source DOI: 10.1128/iai.26.1.173-177.1979 PMC Full Text View journal details

Analysis Services

Isolation and partial characterization of two different heat-stable enterotoxins produced by bovine and porcine strains of enterotoxigenic Escherichia coli.

Contact

WeChat Official Account