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PMID: 387748 Published · ppublish English Journal Article

Selection for Escherichia coli mutants with proteins missing from the ribosome.

Journal of bacteriology ·Vol. 140 ·No. 2 ·1979-11-00 ·Pages 734-7

Dabbs ER

Abstract

Antibiotic-independent revertants of an erythromycin-dependent strain of Escherichia coli were isolated by spontaneous selection. Their ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. In contrast to most ribosomally targeted selections, the specific absence of a certain protein from the ribosome, rather than alterations in ribosomal proteins, was observed. Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing.

MeSH Terms
Bacterial Proteins/analysis,genetics Escherichia coli/analysis,genetics Genes Mutation Ribosomal Proteins/analysis,genetics
Chemicals
Bacterial Proteins Ribosomal Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Dabbs E R
References (12)
12 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.140.2.734-737.1979
Published
1979-11-00
Pages
734-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216704
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.140.2.734-737.1979 PMC Full Text View journal details
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