Home LiteratureArticle Details
PMID: 3877932 Published · ppublish English Journal Article

Bacterial expression of the acquired immunodeficiency syndrome retrovirus p24 gag protein and its use as a diagnostic reagent.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 22 ·1985-11-00 ·Pages 7748-52

Dowbenko DJ, Bell JR, Benton CV, Groopman JE, Nguyen H, Vetterlein D, Capon DJ, Lasky LA

Abstract

A retrovirus [lymphoadenopathy-associated virus, human T-cell leukemia virus type III, acquired immunodeficiency syndrome (AIDS)-related virus] suspected of causing AIDS has been isolated recently. The detection of exposure to this retrovirus in donors of various blood products is important to prevent transmission of the disease from these donors to recipients. In the majority of cases, the detection of antibodies directed against either the viral core protein, a Mr approximately equal to 24,000 protein termed p24 gag, or the viral envelope antigen is proof of previous viral infection. Thus, we have expressed the p24 gag antigen in Escherichia coli in order to produce a diagnostic reagent for the detection of virus exposure. The bacterially synthesized antigen reacts with human and rabbit antisera directed against the native p24 gag protein in both electrophoretic transfer blot assay and ELISA. In addition, the use of bacterially produced antigens for ELISAs gave results that were comparable to those obtained by using antigens isolated from the virus.

MeSH Terms
Acquired Immunodeficiency Syndrome/diagnosis Amino Acid Sequence Antibodies, Viral/analysis Bacteria/metabolism Base Sequence Enzyme-Linked Immunosorbent Assay Gene Products, gag Humans Indicators and Reagents Retroviridae Proteins/analysis,biosynthesis,immunology
Chemicals
Antibodies, Viral Gene Products, gag Indicators and Reagents Retroviridae Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Dowbenko D J
Bell J R
Benton C V
Groopman J E
Nguyen H
Vetterlein D
Capon D J
Lasky L A
References (31)
31 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Human T-cell leukemia virus type II: primary structure analysis of the major internal protein, p24 and the nucleic acid binding protein, p15.
    Virology. 1985 Apr 15;142(1):206-10 PMID: 2997980
  3. Localization of RNA tumor virus polypeptides. I. Isolation of further virus substructures.
    Virology. 1973 Dec;56(2):549-64 PMID: 4127970
  4. Conjugation of antibodies with fluorochromes: modifications to the standard methods.
    J Immunol Methods. 1976;13(3-4):215-26 PMID: 796385
  5. Amino-terminal sequence of bovine leukemia virus major internal protein: homology with mammalian type C virus p30 structural proteins.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2996-3000 PMID: 223166
  6. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  7. The binding of an avian myeloblastosis virus basic 12,000 dalton protein to nucleic acids.
    Nucleic Acids Res. 1979 Dec 11;7(7):2055-72 PMID: 231768
  8. A gas-liquid solid phase peptide and protein sequenator.
    J Biol Chem. 1981 Aug 10;256(15):7990-7 PMID: 7263636
  9. Nucleotide sequence of Moloney murine leukaemia virus.
    Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
  10. Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: evidence of a new acquired cellular immunodeficiency.
    N Engl J Med. 1981 Dec 10;305(24):1425-31 PMID: 6272109
  11. An outbreak of community-acquired Pneumocystis carinii pneumonia: initial manifestation of cellular immune dysfunction.
    N Engl J Med. 1981 Dec 10;305(24):1431-8 PMID: 6975437
  12. Severe acquired immunodeficiency in male homosexuals, manifested by chronic perianal ulcerative herpes simplex lesions.
    N Engl J Med. 1981 Dec 10;305(24):1439-44 PMID: 6272110
  13. Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine.
    Science. 1981 Dec 4;214(4525):1125-9 PMID: 6272395
  14. Primary structure analysis of the major internal protein p24 of human type C T-cell leukemia virus.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1291-4 PMID: 6280175
  15. Nucleotide sequence of Rous sarcoma virus.
    Cell. 1983 Mar;32(3):853-69 PMID: 6299578
  16. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).
    Science. 1983 May 20;220(4599):868-71 PMID: 6189183
  17. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618-22 PMID: 6304725
  18. Transfusion-associated AIDS: serologic evidence of human T-cell leukemia virus infection of donors.
    Science. 1984 Mar 23;223(4642):1309-12 PMID: 6322301
  19. Isolation of new lymphotropic retrovirus from two siblings with haemophilia B, one with AIDS.
    Lancet. 1984 Apr 7;1(8380):753-7 PMID: 6143082
  20. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.
    Science. 1984 May 4;224(4648):500-3 PMID: 6200936
  21. Serological analysis of a subgroup of human T-lymphotropic retroviruses (HTLV-III) associated with AIDS.
    Science. 1984 May 4;224(4648):503-5 PMID: 6200937
  22. Antibodies reactive with human T-lymphotropic retroviruses (HTLV-III) in the serum of patients with AIDS.
    Science. 1984 May 4;224(4648):506-8 PMID: 6324345
  23. Initiation of translation at internal AUG codons in mammalian cells.
    Nature. 1984 May 3-9;309(5963):82-5 PMID: 6717585
  24. Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS.
    Science. 1984 Aug 24;225(4664):840-2 PMID: 6206563
  25. Recovery and inactivation of infectious retroviruses from factor VIII concentration.
    Lancet. 1984 Sep 29;2(8405):722-3 PMID: 6148474
  26. Aetiology of AIDS--antibodies to human T-cell leukaemia virus (type III) in haemophiliacs.
    Nature. 1984 Nov 22-28;312(5992):367-9 PMID: 6095094
  27. Nucleotide sequence and expression of an AIDS-associated retrovirus (ARV-2).
    Science. 1985 Feb 1;227(4686):484-92 PMID: 2578227
  28. Nucleotide sequence of the AIDS virus, LAV.
    Cell. 1985 Jan;40(1):9-17 PMID: 2981635
  29. Complete nucleotide sequence of the AIDS virus, HTLV-III.
    Nature. 1985 Jan 24-30;313(6000):277-84 PMID: 2578615
  30. Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus.
    Nature. 1985 Feb 7-13;313(6002):450-8 PMID: 2982104
  31. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.
    Immunochemistry. 1971 Sep;8(9):871-4 PMID: 5135623
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.22.7748
Published
1985-11-00
Pages
7748-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391411
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.22.7748 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2