Home LiteratureArticle Details
PMID: 3882663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli.

Journal of bacteriology ·Vol. 161 ·No. 3 ·1985-03-00 ·Pages 1059-68

Rule GS, Pratt EA, Chin CC, Wold F, Ho C

Abstract

Recombinant DNA plasmids containing the gene for the membrane-bound D-lactate dehydrogenase (D-LDH) of Escherichia coli linked to the promoter PL from lambda were constructed. After induction, the levels of D-LDH were elevated 300-fold over that of the wild type and amounted to 35% of the total cellular protein. The nucleotide sequence of the D-LDH gene was determined and shown to agree with the amino acid composition and the amino-terminal sequence of the purified enzyme. Removal of the amino-terminal formyl-Met from D-LDH was not inhibited in cells which contained these high levels of D-LDH.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Membrane/enzymology Codon Enzyme Induction Escherichia coli/enzymology,genetics Genes, Bacterial L-Lactate Dehydrogenase/biosynthesis,genetics Plasmids
Chemicals
Codon L-Lactate Dehydrogenase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rule G S
Pratt E A
Chin C C
Wold F
Ho C
References (41)
41 references, click to expand
  1. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  2. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.
    Gene. 1982 Jun;18(3):199-209 PMID: 6751939
  3. A biochemical study of the reconstitution of D-lactate dehydrogenase-deficient membrane vesicles using fluorine-labeled components.
    Biochim Biophys Acta. 1983 Apr 6;729(2):167-75 PMID: 6338924
  4. Cloning of the gene for the respiratory D-lactate dehydrogenase of Escherichia coli.
    Biochemistry. 1982 Apr 27;21(9):2092-5 PMID: 7046794
  5. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  6. Overproduction of fumarate reductase in Escherichia coli induces a novel intracellular lipid-protein organelle.
    J Bacteriol. 1984 May;158(2):590-6 PMID: 6373722
  7. Amplification of the respiratory NADH dehydrogenase of Escherichia coli by gene cloning.
    Gene. 1978 Sep;4(1):25-36 PMID: 365690
  8. Membrane D-lactate dehydrogenase from Escherichia coli. Purification and properties.
    Biochemistry. 1973 Jun 19;12(13):2468-74 PMID: 4575624
  9. Genetic identification and purification of the respiratory NADH dehydrogenase of Escherichia coli.
    Biochemistry. 1981 Mar 31;20(7):2041-7 PMID: 6784762
  10. Amino acid sequence for the peptide extension on the prolipoprotein of the Escherichia coli outer membrane.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1004-8 PMID: 322142
  11. Termination of transcription in E. coli.
    Cell. 1983 Apr;32(4):1029-32 PMID: 6188534
  12. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
    J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
  13. Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon.
    Eur J Biochem. 1981 May;116(1):165-70 PMID: 6265208
  14. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  15. Use of the lambda phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles.
    Methods Enzymol. 1979;68:482-92 PMID: 232223
  16. ENZYME INDUCTION AS AN ALL-OR-NONE PHENOMENON.
    Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):553-66 PMID: 16590055
  17. pUR222, a vector for cloning and rapid chemical sequencing of DNA.
    Nucleic Acids Res. 1981 Aug 25;9(16):4087-98 PMID: 6272193
  18. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
    J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
  19. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6012-6 PMID: 366607
  20. The amino acid sequence of the delta haemolysin of Staphylococcus aureus.
    FEBS Lett. 1980 Jun 30;115(2):209-12 PMID: 7398877
  21. D-lactate dehydrogenase binding in Escherichia coli dld- membrane vesicles reconstituted for active transport.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1461-5 PMID: 4598306
  22. Lipid dependence of the membrane-bound D-lactate dehydrogenase of Escherichia coli.
    J Biol Chem. 1981 Oct 25;256(20):10369-74 PMID: 7026558
  23. Exonuclease III: use for DNA sequence analysis and in specific deletions of nucleotides.
    Methods Enzymol. 1983;100:60-96 PMID: 6312265
  24. Characterization of translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
  25. Mechanisms of active transport in isolated bacterial membrane vesicles. XV. Purification and properties of the membrane-bound D-lactate dehydrogenase from Escherichia coli.
    J Biol Chem. 1973 Oct 25;248(20):7012-7 PMID: 4582730
  26. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  27. Amino-terminally formylated tryptophan synthase alpha-subunit produced by the trp operon cloned in a plasmid vector.
    J Biochem. 1980 Jan;87(1):351-4 PMID: 6987211
  28. Plasmids of Escherichia coli as cloning vectors.
    Methods Enzymol. 1979;68:245-67 PMID: 232214
  29. In vitro synthesis of the membrane-bound D-lactate dehydrogenase of Escherichia coli.
    Biochemistry. 1982 Apr 27;21(9):2085-91 PMID: 7046793
  30. Structure of the lac carrier protein of Escherichia coli.
    J Biol Chem. 1983 Jan 10;258(1):31-4 PMID: 6336750
  31. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  32. A rapid alkaline extraction method for the isolation of plasmid DNA.
    Methods Enzymol. 1983;100:243-55 PMID: 6353143
  33. Localization of D-lactate dehydrogenase in membrane vesicles prepared by using a french press or ethylenediaminetetraacetate-lysozyme from Escherichia coli.
    J Bacteriol. 1975 Oct;124(1):470-5 PMID: 809422
  34. Fine structure of Myxococcus xanthus during morphogenesis.
    J Bacteriol. 1962 Nov;84:943-52 PMID: 13997609
  35. Reconstitution of transport dependent on D-lactate or glycerol 3-phosphate in membrane vesicles of Escherichia coli deficient in the corresponding dehydrogenases.
    Biochemistry. 1974 May 21;13(11):2327-33 PMID: 4598623
  36. Membrane-bound D-lactate dehydrogenase from Escherichia coli: purification and properties.
    Biochemistry. 1979 Jan 23;18(2):312-6 PMID: 369599
  37. Amino acid sequence of the proteolipid subunit of the proton-translocating ATPase complex from the thermophilic bacterium PS-3.
    Eur J Biochem. 1980;107(1):57-65 PMID: 6447066
  38. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  39. Initiation of E. coli proteins.
    Proc Natl Acad Sci U S A. 1966 Jun;55(6):1517-24 PMID: 5336288
  40. Mechanisms of active transport in isolated membrane vesicles. I. The site of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in Escherichia coli membrane vesicles.
    J Biol Chem. 1971 Sep 10;246(17):5518-22 PMID: 4330922
  41. Characterization of the respiratory NADH dehydrogenase of Escherichia coli and reconstitution of NADH oxidase in ndh mutant membrane vesicles.
    Biochemistry. 1981 Jun 9;20(12):3621-8 PMID: 7020757
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.3.1059-1068.1985
Published
1985-03-00
Pages
1059-68
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215007
Subset
IM
Grants
NIGMS NIH HHS · GM-08067 · United States
NIGMS NIH HHS · GM-26874 · United States
NIGMS NIH HHS · GM-31305 · United States
Databases
GENBANK
M10038
External Links
PubMed source DOI: 10.1128/jb.161.3.1059-1068.1985 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2