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PMID: 3883989 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Effect of insulin on glucose transport and metabolism in isolated fat-cells of gonadal adipose tissue from mature age-matched male and female rats.

The Biochemical journal ·Vol. 225 ·No. 2 ·1985-01-15 ·Pages 343-8

Guerre-Millo M, Leturque A, Lavau M, Girard J

Abstract

Insulin action on glucose transport and metabolism was studied in paraovarian adipocytes from 3-month-old female rats and compared with insulin action in epididymal adipocytes from closely age-matched males. At maximal insulin concentrations the stimulations of 2-deoxyglucose uptake (4-fold the basal value) and of [U-14C]glucose incorporation into CO2 and total lipids (3- and 2-fold the basal values respectively) were similar in adipocytes from rats of both sexes. At submaximal insulin concentrations (less than 0.2 nM) the ability of paraovarian adipocytes to transport and to metabolize glucose was higher than that of epididymal adipocytes; accordingly an increase in insulin binding was observed in paraovarian adipocytes as compared with epididymal adipocytes. These results show that paraovarian adipocytes from mature female rats were highly responsive to insulin, and exhibited a higher sensitivity to the hormone than did epididymal adipocytes from male rats of the same age.

MeSH Terms
Adipose Tissue/cytology,drug effects,metabolism Animals Biological Transport/drug effects Deoxyglucose/metabolism Female Glucose/metabolism Gonads/drug effects,metabolism Insulin/pharmacology Lipid Metabolism Male Rats Rats, Inbred Strains Sex Factors
Chemicals
Insulin Deoxyglucose Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guerre-Millo M
Leturque A
Lavau M
Girard J
References (31)
31 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2250343
Published
1985-01-15
Pages
343-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144596
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2250343 PMC Full Text View journal details
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