Home LiteratureArticle Details
PMID: 388428 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

RNA polymerase III transcriptional units are interspersed among human non-alpha-globin genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 10 ·1979-10-00 ·Pages 5095-9

Duncan C, Biro PA, Choudary PV, Elder JT, Wang RR, Forget BG, de Riel JK, Weissman SM

Abstract

Cloned human DNA fragments containing globin genes are transcribed in vitro to form discrete RNA species. One transcription unit is located approximately 1500 base pairs upstream from the G-gamma-globin gene. This transcript is partially homologous to a polymerase III template located approximately 1000 base pairs upstream from the delta-globin gene and to DNA located a short distance downstream from the beta-globin gene.

MeSH Terms
Cloning, Molecular DNA, Recombinant/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/metabolism Genes Globins/biosynthesis HeLa Cells/metabolism Humans Plasmids RNA Polymerase III/metabolism Transcription, Genetic
Chemicals
DNA, Recombinant Globins DNA-Directed RNA Polymerases RNA Polymerase III
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Duncan C
Biro P A
Choudary P V
Elder J T
Wang R R
Forget B G
de Riel J K
Weissman S M
References (21)
21 references, click to expand
  1. Structure of the human fetal globin gene locus.
    Nature. 1979 Mar 15;278(5701):227-31 PMID: 423971
  2. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
    Cell. 1978 Dec;15(4):1157-74 PMID: 728996
  3. Comparison of cloned mouse alpha- and beta-globin genes: conservation of intervening sequence locations and extragenic homology.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6187-91 PMID: 282635
  4. Restriction endonuclease mapping of the human gamma globin gene loci.
    Nucleic Acids Res. 1979 Jun 11;6(7):2519-44 PMID: 461196
  5. Analysis of the beta-delta-globin gene loci in normal and Hb Lepore DNA: direct determination of gene linkage and intergene distance.
    Cell. 1978 Sep;15(1):25-41 PMID: 699045
  6. Organization of human delta--and beta-globin genes in cellular DNA and the presence of intragenic inserts.
    Cell. 1978 Sep;15(1):15-23 PMID: 699038
  7. Characterisation of deletions which affect the expression of fetal globin genes in man.
    Nature. 1979 Jun 14;279(5714):598-603 PMID: 450109
  8. Transcriptional role of yeast deoxyribonucleic acid dependent ribonucleic acid polymerase III.
    Biochemistry. 1978 Feb 21;17(4):750-8 PMID: 341977
  9. Adenovirus DNA-directed transcription of 5.5S RNA in vitro.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2175-9 PMID: 276858
  10. Identification of precursor molecules to individual tRNA species from Bombyx mori.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):635-9 PMID: 273226
  11. Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.
    Cell. 1978 Dec;15(4):1145-56 PMID: 264240
  12. Low molecular weight RNAs hydrogen-bonded to nuclear and cytoplasmic poly(A)-terminated RNA from cultured Chinese hamster ovary cells.
    Cell. 1978 Sep;15(1):205-14 PMID: 699042
  13. A general method for the purification of restriction enzymes.
    Nucleic Acids Res. 1978 Jul;5(7):2373-80 PMID: 673857
  14. Biochemical and genetic properties of site-specific restriction endonucleases in Bacillus globigii.
    J Bacteriol. 1978 Apr;134(1):338-44 PMID: 649568
  15. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
    Cell. 1974 Dec;3(4):315-25 PMID: 4216403
  16. Electrophoresis of RNA in formamide.
    Biochemistry. 1974 Dec 17;13(26):5373-8 PMID: 4611484
  17. Nuclear RNA metabolism.
    Annu Rev Biochem. 1973;42:329-54 PMID: 4581227
  18. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  19. Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences.
    Cell. 1976 Aug;8(4):479-84 PMID: 954101
  20. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  21. Studies on the origin of a low molecular weight ribonucleic acid from human cells infected with adenoviruses.
    J Biol Chem. 1969 Oct 10;244(19):5320-32 PMID: 5344138
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.10.5095
Published
1979-10-00
Pages
5095-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC413086
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.10.5095 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2