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PMID: 388430 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Termination of transcription by bacteriophage T3 RNA polymerase: homogeneous 3'-terminal oligonucleotide sequence of in vitro T3 RNA polymerase transcripts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 10 ·1979-10-00 ·Pages 5110-3

Majumder HK, Maitra U, Rosenberg M

Abstract

RNA was synthesized in vitro from a T3 DNA template by T3 RNA polymerase and subsequently separated into seven discrete size classes (molecular weights ranging between 0.21 x 10(6) and 6.2 x 10(6)) by electrophoresis in polyacrylamide slab gels. RNase T1-generated 3'-terminal oligonucleotide fragments were then selectively isolated from either the unfractionated total RNA or the gel-purified specific transcripts by chromatography on columns of dihydroxyboryl-cellulose. Sequence analysis of these oligonucleotide products indicated that the unfractionated transcripts as well as all the individual major RNA species examined had a unique sequence, (Gp)UpUpUpUpUpGOH, at their 3' termini. The specificity of this sequence, as well as the total lack of any sequence heterogeneity at the ends of these transcripts, indicates a high degree of specificity of termination during transcription in this system.

MeSH Terms
Base Sequence DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Oligoribonucleotides/analysis T-Phages/enzymology Transcription, Genetic
Chemicals
Oligoribonucleotides DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Majumder H K
Maitra U
Rosenberg M
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.10.5110
Published
1979-10-00
Pages
5110-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC413089
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.10.5110 PMC Full Text View journal details
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