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PMID: 3885846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and expression of Staphylococcus aureus plasmid-mediated quaternary ammonium resistance in Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 27 ·No. 1 ·1985-01-00 ·Pages 79-83

Tennent JM, Lyon BR, Gillespie MT, May JW, Skurray RA

Abstract

The Staphylococcus aureus plasmid pSK1 carries Tn4001, a 4.7-kilobase (kb) transposon which specifies resistance to gentamicin, tobramycin, and kanamycin. In addition, pSK1 mediates resistance to trimethoprim and linked resistance to ethidium bromide (Ebr) and to quaternary ammonium compounds (Qar). Restriction endonuclease analysis of pSK1 and a deleted derivative of pSK1 revealed that the gene(s) responsible for Ebr Qar lies within a 5.2-kb HindIII fragment. This fragment has been cloned into the Escherichia coli plasmid vector pBR322, and transformants of an E. coli K-12 strain exhibited Ebr Qar. Subcloning of the 5.2-kb insert, combined with data from electron microscopic analysis of deleted derivatives of pSK1, located the Ebr Qar determinant(s) on a 2.3-kb segment of pSK1 DNA.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular DNA, Bacterial/biosynthesis Escherichia coli/drug effects,genetics,ultrastructure Quaternary Ammonium Compounds/pharmacology R Factors Staphylococcus aureus/genetics Transformation, Bacterial
Chemicals
DNA, Bacterial Quaternary Ammonium Compounds
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tennent J M
Lyon B R
Gillespie M T
May J W
Skurray R A
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14 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.27.1.79
Published
1985-01-00
Pages
79-83
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC176209
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.27.1.79 PMC Full Text View journal details
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