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PMID: 3885942 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Expression of the cloned subunits of bacterial luciferase from separate replicons.

Biochemical and biophysical research communications ·Vol. 127 ·No. 3 ·1985-03-29 ·Pages 1007-11

Gupta SC, O'Brien D, Hastings JW

Abstract

The lux A and lux B genes of Vibrio harveyi, encoding the alpha and beta subunits of bacterial luciferase, were cloned individually into Escherichia coli in two different compatible plasmids. Active luciferase was formed in an amount equal to that produced in cells carrying a plasmid with the cloned genes on a single fragment.

MeSH Terms
DNA, Recombinant Escherichia coli/genetics Genes, Bacterial Luciferases/genetics Luminescent Measurements Plasmids Protein Biosynthesis Transcription, Genetic Vibrio/enzymology,genetics
Chemicals
DNA, Recombinant Luciferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gupta S C
O'Brien D
Hastings J W
Article Info
Journal
Biochemical and biophysical research communications
Abbr.
Biochem Biophys Res Commun
ISSN
0006-291X
DOI
10.1016/s0006-291x(85)80044-9
Published
1985-03-29
Pages
1007-11
Language
English
Region
United States
NLM ID
0372516
Subset
IM
External Links
PubMed source DOI: 10.1016/s0006-291x(85)80044-9 View journal details
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