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PMID: 3886543 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immunochemistry of the Streptococcus mutans BHT cell membrane: detection of determinants cross-reactive with human heart tissue.

Infection and immunity ·Vol. 48 ·No. 2 ·1985-05-00 ·Pages 280-6

Ayakawa GY, Siegel JL, Crowley PJ, Bleiweis AS

Abstract

Cell membranes of Streptococcus mutans BHT serotype b were prepared after glass bead disruption or mutanolysin digestion of whole cells. Immunoblot analyses of BHT membrane extracts revealed major polypeptides of 42,000, 46,000, 62,000, and 82,000 daltons, as well as several minor bands, to be reactive with rabbit anti-human heart immunoglobulins. Heart cross-reactive antigens have been reported in the cell walls and culture fluids of several S. mutans serotypes. This represents the first report of cell membrane-localized heart cross-reactive antigens in this oral pathogen. Positive enzyme-linked immunosorbent assay and immunoblot reactions were also obtained with heart tissue antigen and anti-BHT sera, indicating mutual cross-reactivity. The major cross-reactive component detected by immunoblotting of human heart extracts was a 69,000-dalton polypeptide.

MeSH Terms
Antigens, Bacterial/immunology Antigens, Surface/immunology Bacterial Proteins/immunology Cross Reactions Enzyme-Linked Immunosorbent Assay Humans Immunologic Techniques Molecular Weight Myocardium/immunology Streptococcus mutans/immunology
Chemicals
Antigens, Bacterial Antigens, Surface Bacterial Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ayakawa G Y
Siegel J L
Crowley P J
Bleiweis A S
References (28)
28 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Antigens of Streptococcus mutans. I. Characterization of a serotype-specific determinant from Streptococcus mutans.
    Infect Immun. 1973 May;7(5):795-804 PMID: 4128669
  3. Growth of several cariogenic strains of oral streptococci in a chemically defined medium.
    Infect Immun. 1975 Apr;11(4):649-55 PMID: 1091546
  4. Immunization against dental caries.
    Br Dent J. 1975 Jul 15;139(2):45-58 PMID: 806292
  5. Group A streptococcal antigens cross-reactive with myocardium. Purification of heart-reactive antibody and isolation and characterization of the streptococcal antigen.
    J Exp Med. 1977 Aug 1;146(2):579-99 PMID: 327018
  6. Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype c.
    Arch Oral Biol. 1978;23(1):7-15 PMID: 273396
  7. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  8. Wall-associated protein antigens of Streptococcus mutans.
    J Gen Microbiol. 1979 Sep;114(1):109-15 PMID: 118231
  9. Immunisation with a purified protein from Streptococcus mutans against dental caries in rhesus monkeys.
    Lancet. 1980 May 10;1(8176):995-6 PMID: 6103387
  10. Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue.
    Infect Immun. 1980 Feb;27(2):576-88 PMID: 6991419
  11. Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component.
    Infect Immun. 1980 May;28(2):486-93 PMID: 6995311
  12. Cross-reactivity of Streptococcus mutans antigens and human heart tissue.
    Infect Immun. 1980 Oct;30(1):69-73 PMID: 6160105
  13. Distribution of cross-reactive antigens A and B in Streptococcus mutans and other oral streptococci.
    J Gen Microbiol. 1980 Jun;118(2):383-8 PMID: 7441199
  14. Mutanolysin-induced spheroplasts of Streptococcus mutants are true protoplasts.
    Infect Immun. 1981 Feb;31(2):808-15 PMID: 7012022
  15. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  16. Immunobiology of dental caries: microbial aspects and local immunity.
    Annu Rev Microbiol. 1981;35:595-638 PMID: 7027907
  17. Isolation of heart- and kidney-binding protein from group A streptococci.
    Infect Immun. 1982 Jan;35(1):335-42 PMID: 7033140
  18. Separation and characterization of a protein antigen from cells of Streptococcus mutans.
    J Gen Microbiol. 1981 Feb;122(2):217-25 PMID: 6459427
  19. Immunisation of monkeys (Macaca fascicularis) with antigens purified from Streptococcus mutans.
    Br Dent J. 1982 Feb 2;152(3):81-4 PMID: 6949599
  20. Examination of Streptococcus mutans for immunoglobulin G Fc reactivity.
    Infect Immun. 1981 Dec;34(3):851-5 PMID: 7333670
  21. Glucokinase from Streptococcus mutans.
    Methods Enzymol. 1982;90 Pt E:25-30 PMID: 7154950
  22. Detection of receptors for the Fc region of IgG on streptococci.
    J Immunol Methods. 1983 Apr 15;59(1):83-94 PMID: 6187866
  23. Lack of antibodies to human heart tissue in sera of rhesus monkeys immunized with Streptococcus mutans antigens and comparative study with rabbit antisera.
    Infect Immun. 1983 Jun;40(3):1075-82 PMID: 6852912
  24. Binding of Todd-Hewitt broth antigens by Streptococcus mutans.
    Infect Immun. 1983 Jun;40(3):1140-5 PMID: 6852915
  25. Characteristics of a high molecular weight extracellular protein of Streptococcus mutans.
    J Gen Microbiol. 1983 Sep;129(9):2779-88 PMID: 6415234
  26. Serology and tissue lesions in rabbits immunized with Streptococcus mutans.
    J Immunol. 1983 Dec;131(6):3021-7 PMID: 6417238
  27. Chemical analyses of membranes isolated from Streptococcus mutans BHT.
    J Dent Res. 1984 Dec;63(12):1343-7 PMID: 6392377
  28. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.48.2.280-286.1985
Published
1985-05-00
Pages
280-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261301
Subset
IM
Grants
NIDCR NIH HHS · DE06051 · United States
External Links
PubMed source DOI: 10.1128/iai.48.2.280-286.1985 PMC Full Text View journal details
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