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PMID: 3888112 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cloning and expression in Escherichia coli of pectinase genes of Erwinia carotovora subsp. carotovora.

Applied and environmental microbiology ·Vol. 49 ·No. 3 ·1985-03-00 ·Pages 714-7

Zink RT, Chatterjee AK

Abstract

Genes coding for an endo-pectate lyase, an exo-pectate lyase, and an endopolygalacturonase of Erwinia carotovora subsp. carotovora Ecc71 were cloned in Escherichia coli HB101, using the cosmid pHC79. The products of the cloned pectinase genes paralleled their counterparts in strain Ecc71 in isoelectric mobility, mode of substrate degradation, and ability to macerate potato tuber tissue.

MeSH Terms
Cloning, Molecular Erwinia/enzymology,genetics Escherichia coli/genetics Glycoside Hydrolases/genetics Polygalacturonase/genetics
Chemicals
Glycoside Hydrolases Polygalacturonase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zink R T
Chatterjee A K
References (13)
13 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.49.3.714-717.1985
Published
1985-03-00
Pages
714-7
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC373578
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.49.3.714-717.1985 PMC Full Text View journal details
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