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PMID: 3889921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 12 ·1985-06-00 ·Pages 4055-9

Reed SI, Hadwiger JA, Lörincz AT

Abstract

Antibodies raised against the protein encoded by a lacZ-CDC28 in-frame fusion were shown to immunoprecipitate the CDC28 product from yeast cell lysates. The polypeptide p36CDC28 is a phosphoprotein of apparent Mr 36,000. Immune complexes prepared from yeast cell lysates by using anti-CDC28 antibody were found to possess a protein kinase activity, as determined by the transfer of label from [gamma-32P]ATP to a coprecipitated Mr 40,000 protein of unknown identity or function (p40). This activity was absent or thermolabile when extracts were prepared from several different cdc28 temperature-sensitive strains. The protein kinase activity was dependent on Zn2+ and transferred phosphate specifically to serine and threonine residues.

MeSH Terms
Amino Acid Sequence Antigen-Antibody Complex Cell Cycle Fungal Proteins/metabolism Isoelectric Point Molecular Weight Mutation Phosphoproteins/physiology Phosphorylation Protein Kinases/genetics,immunology Saccharomyces cerevisiae/enzymology,genetics Temperature
Chemicals
Antigen-Antibody Complex Fungal Proteins Phosphoproteins Protein Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reed S I
Hadwiger J A
Lörincz A T
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16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.12.4055
Published
1985-06-00
Pages
4055-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397933
Subset
IM
Grants
NIGMS NIH HHS · GM28005 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.12.4055 PMC Full Text View journal details
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