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PMID: 3894933 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.

Molecular and cellular biology ·Vol. 5 ·No. 7 ·1985-07-00 ·Pages 1601-10

Harlow E, Williamson NM, Ralston R, Helfman DM, Adams TE

Abstract

Three clones for the human tumor antigen p53 were isolated from a cDNA library prepared from A431 cells. One of these clones, pR4-2, contains the entire coding region for human p53. This clone directs the synthesis of a polypeptide with the correct molecular weight and immunological epitopes of an authentic p53 molecule in an in vitro transcription-translation reaction. Although the pR4-2 clone contains the coding region for p53, it is not a full-length copy of the human p53 mRNA. Northern analysis showed that the p53 mRNA is approximately 2,500 nucleotides long, whereas the pR4-2 insert is only 1,760 base pairs in length. Analysis of the DNA sequence of this clone suggests that the human p53 polypeptide has 393 amino acids. We compared the predicted amino acid sequence of the pR4-2 clone with similar clones for the mouse p53 and found long regions of amino acid homology between these two molecules.

MeSH Terms
Amino Acid Sequence Animals Antigens, Neoplasm/genetics Base Sequence Cell Line Cloning, Molecular DNA/genetics Female Humans Mice Molecular Weight Neoplasm Proteins/genetics Phosphoproteins/genetics Tumor Suppressor Protein p53
Chemicals
Antigens, Neoplasm Neoplasm Proteins Phosphoproteins Tumor Suppressor Protein p53 DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Harlow E
Williamson N M
Ralston R
Helfman D M
Adams T E
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50 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.7.1601-1610.1985
Published
1985-07-00
Pages
1601-10
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367278
Subset
IM
Grants
NCI NIH HHS · CA13106 · United States
Databases
GENBANK
K03199
External Links
PubMed source DOI: 10.1128/mcb.5.7.1601-1610.1985 PMC Full Text View journal details
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