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PMID: 3895226 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Solubilization of a vectorial transmembrane receptor in functional form: aspartate receptor of chemotaxis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 15 ·1985-08-00 ·Pages 4891-5

Bogonez E, Koshland DE

Abstract

The aspartate receptor, an integral membrane protein in the bacterial chemosensory system, has been solubilized in functional form by a combination of detergent, phospholipid, and glycerol. The conformation of the solubilized receptor is the same as that of the protein in vivo, as indicated by aspartate binding, rates of methyl esterification, and quantitative correlation of stimulus with this covalent modification. Studying the functional solubilized receptor in a homogeneous solution avoids many difficulties associated with an in vivo or a vesicle-reconstituted receptor. The technique of adding lipids, detergent, and glycerol to solubilize the protein in active form appears to be generally applicable.

MeSH Terms
Aspartic Acid/physiology Bacterial Proteins/isolation & purification,physiology Chemoreceptor Cells/physiology Chemotaxis Hydrogen-Ion Concentration Kinetics Membrane Proteins/isolation & purification,physiology Methylation Phospholipids/pharmacology Protein Conformation Salmonella typhimurium/physiology Solubility Structure-Activity Relationship
Chemicals
Bacterial Proteins Membrane Proteins Phospholipids Aspartic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bogonez E
Koshland D E
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.15.4891
Published
1985-08-00
Pages
4891-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390463
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.15.4891 PMC Full Text View journal details
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