Capping of exogenous Forssman glycolipid on cells.

The Journal of cell biology ·Vol. 82 ·No. 3 ·1979-09-00 ·Pages 829-33

Stern PL, Bretscher MS

Abstract

When motile cells are incubated with Forssman glycolipid, the antigen is incorporated into the cells' plasma membranes. If cross-linked by antibody, the patched glycolipids cap. This process is sensitive to those drugs that are known to inhibit capping of protein antigens. The results support a flow mechanism for capping.

MeSH Terms

Animals Cell Line Cell Membrane/immunology Colchicine/pharmacology Cytochalasin B/pharmacology Dinitrophenols/pharmacology Fluorescent Antibody Technique Forssman Antigen/immunology Glycolipids/immunology Immunologic Capping/drug effects Mice Neoplasms T-Lymphocytes/immunology

Chemicals

Dinitrophenols Glycolipids Cytochalasin B Forssman Antigen Colchicine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stern P L

Bretscher M S

References (10)

10 references, click to expand

Transmembrane interactions and the mechanism of capping of surface receptors by their specific ligands.
Proc Natl Acad Sci U S A. 1977 Nov;74(11):5031-5 PMID: 337308
Nonuniform distribution of concanavalin-A receptors and surface antigens on uropod-forming thymocytes.
J Cell Biol. 1978 Oct;79(1):235-51 PMID: 308949
Monoclonal antibodies as probes for differentiation and tumor-associated antigens: a Forssman specificity on teratocarcinoma stem cells.
Cell. 1978 Aug;14(4):775-83 PMID: 567532
Normal distribution, patching and capping of lymphocyte surface immunoglobulin studied by electron microscopy.
Nat New Biol. 1973 Feb 28;241(113):257-9 PMID: 4540373
An improved isolation method and new composition data for Forssman hapten from sheep erythrocytes.
Immunochemistry. 1973 Nov;10(11):745-53 PMID: 4358346
Mobility of cholera toxin receptors on rat lymphocyte membranes.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3844-8 PMID: 1060063
Ligand-induced redistribution of lymphocyte membrane ganglioside GM1.
Nature. 1975 Sep 11;257(5522):103-6 PMID: 1057731
Directed lipid flow in cell membranes.
Nature. 1976 Mar 4;260(5546):21-3 PMID: 1264188
Sheep erythrocyte Forssman hapten, an isohapten system: composition of the ceramide.
Immunochemistry. 1975 Apr;12(4):297-302 PMID: 1193672
The locomotion of fibroblasts in culture. 3. Movements of particles on the dorsal surface of the leading lamella.
Exp Cell Res. 1970 Oct;62(2):389-98 PMID: 5531377

Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.82.3.829

Published

1979-09-00

Pages

829-33

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2110488

Subset

External Links

PubMed source DOI: 10.1083/jcb.82.3.829 PMC Full Text View journal details

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Capping of exogenous Forssman glycolipid on cells.

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