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PMID: 389953 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Formalinized Chlamydia trachomatis organisms as antigen in the micro-immunofluorescence test.

Journal of clinical microbiology ·Vol. 10 ·No. 2 ·1979-08-00 ·Pages 259-61

Wang SP, Kuo CC, Grayston JT

Abstract

Chlamydia trachomatis organisms grown in HeLa 229 cell cultures were purified and formalinized for use in the micro-immunofluorescence test. As test antigens, they were stable when stored unfrozen at 4 degrees C for a long period of time without loss of type specificity and sensitivity.

MeSH Terms

Antigens, Bacterial/immunology Chlamydia Infections/diagnosis Chlamydia trachomatis/drug effects,growth & development,immunology Diagnosis, Differential Fluorescent Antibody Technique Formaldehyde/pharmacology HeLa Cells Humans Temperature

Chemicals

Antigens, Bacterial Formaldehyde

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Wang S P

Kuo C C

Grayston J T

References (8)

8 references, click to expand

Modification of the microimmunofluorescence test to provide a routine serodiagnostic test for chlamydial infection.
J Clin Pathol. 1977 Jun;30(6):510-7 PMID: 326816
Immunologic classification of Neisseria gonorrhoeae with micro-immunofluorescence.
J Immunol. 1977 Sep;119(3):795-803 PMID: 408415
TRIC type K, a new immunologic type of Chlamydia trachomatis.
J Immunol. 1974 Aug;113(2):591-6 PMID: 4210884
Three new immunologic types of trachoma-inclusion conjunctivitis organisms.
J Immunol. 1973 Mar;110(3):873-9 PMID: 4631875
Chlamydia trachomatis immunotype J.
J Immunol. 1975 Dec;115(6):1711-6 PMID: 810514
Simplified serological test for antibodies to Chlamydia trachomatis.
J Clin Microbiol. 1976 Jul;4(1):6-10 PMID: 783191
New knowledge of chlamydiae and the diseases they cause.
J Infect Dis. 1975 Jul;132(1):87-105 PMID: 1097546
A potency test for trachoma vaccine utilizing the mouse toxicity prevention test.
Am J Ophthalmol. 1967 May;63(5):Suppl:1443-54 PMID: 6025170

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.10.2.259-261.1979

Published

1979-08-00

Pages

259-61

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC273143

Subset

External Links

PubMed source DOI: 10.1128/jcm.10.2.259-261.1979 PMC Full Text View journal details

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Formalinized Chlamydia trachomatis organisms as antigen in the micro-immunofluorescence test.

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